MAP2K7 Antibody | anti-MAP2K7 antibody

MAP2K7 Antibody

Cat. Num. AAA8516379

• Gene Names: MAP2K7; MEK; MKK7; JNKK2; MEK 7; MAPKK7; PRKMK7; SAPKK4; SAPKK-4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified
• Synonyms: MAP2K7; MAP2K7 Antibody; Jnkk2; MAPKK7; MKK7; PRKMK7; SAPKK-4; SAPKK4; anti-MAP2K7 antibody

• Reactivity: Human, Mouse, Rat
• Specificity: MAP2K7 Antibody detects endogenous levels of total MAP2K7
• Purity/Purification: Immunogen affinity purified
• Form/Format: In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 435

• Applicable Applications for anti-MAP2K7 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human MAP2K7
• Preparation and Storage: Store at-20 degree C for 1 year

Western Blot (WB)

(Western blot analysis of extracts of various celllines, using MAP2K7 antibody.)

Related Product Information for anti-MAP2K7 antibody

c-Jun NH2-terminal protein kinase (JNK), a distant member of the mitogen-activated protein (MAP) kinase family, regulates gene expression in response to various extracellular stimuli. MKK7, a novel member of the MAP kinase kinase family, was phosphorylated and activated by MEKK1, a MAP kinase kinasekinase in the JNK signaling cascade. MKK7 activity was also stimulated by constitutively active forms of Rac and Cdc42Hs, members of the Rho small GTP-binding protein family (1). endogenous MKK7 was activated by treatment with the growth factor interleukin-3 (but not interleukin-4), or by ligation of CD40, the B-cell antigen receptor, or the receptor for the Fc fragment of immunoglobulin. MKK7 was also activated when cells were exposed to heat, UV irradiation, anisomycin, hyperosmolarity or the pro-inflammatory cytokine tumor necrosis factor-alpha, suggesting that MKK7 is involved in many physiological pathways (2).

NCBI and Uniprot Product Information

• NCBI GI #: 662033893
• NCBI GeneID: 5609
• NCBI Accession #: NP_001284484.1
• NCBI GenBank Nucleotide #: NM_001297555.1
• UniProt Accession #: O14733; O14648; O14816; O60452; O60453; Q1PG43; Q8IY10; B2R9S5; D6W659
• Molecular Weight: 47kDa
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 7 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 7
• NCBI Official Symbol: MAP2K7
• NCBI Official Synonym Symbols: MEK; MKK7; JNKK2; MEK 7; MAPKK7; PRKMK7; SAPKK4; SAPKK-4
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 7
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 7
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: MAP2K7
• UniProt Synonym Gene Names: JNKK2; MEK7; MKK7; PRKMK7; SKK4; MAP kinase kinase 7; MAPKK 7; MEK 7; SAPK kinase 4; SAPKK-4; SAPKK4; JNK kinase 2; JNKK 2

NCBI Description

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.