LIMK2 Antibody | anti-LIMK2 antibody

LIMK2 (Phospho-Thr505) Antibody

Cat. Num. AAA859668

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: LIMK2; LIMK2 (Phospho-Thr505) Antibody; anti-LIMK2 antibody

• Reactivity: Human, Mouse, Rat
• Specificity: LIMK2 (phospho-Thr505) antibody detects endogenous levels of LIMK2 only when phosphorylated at threonine 505.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 686

• Applicable Applications for anti-LIMK2 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:10000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human LIMK2 around the phosphorylation site of threonine 505 (R-Y-TP-V-V).
• Preparation and Storage: Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK2 (phospho-Thr505) antibody.)

Western Blot (WB)

(Western blot analysis of the extracts from MCF7 cells using LIMK2 (phospho-Thr505) antibody.)

Product Categories/Family for anti-LIMK2 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Soosairajah J, et al. (2005) EMBO J. Ohashi K, et al. (2000) J Biol Chem; 275(5): 3577-82. Edwards DC, et al. (1999) Nat Cell Biol; 1(5): 253-9.

NCBI and Uniprot Product Information

• NCBI GI #: 73390140
• NCBI GeneID: 3985
• NCBI Accession #: NP_001026971.1
• NCBI GenBank Nucleotide #: NM_001031801.1
• UniProt Accession #: P53671; Q7KZ80; Q7L3H5; Q96E10; Q99464; Q9UFU0; A8K6H5
• Molecular Weight: 77,886 Da
• NCBI Official Full Name: LIM domain kinase 2 isoform 1
• NCBI Official Synonym Full Names: LIM domain kinase 2
• NCBI Official Symbol: LIMK2
• NCBI Protein Information: LIM domain kinase 2
• UniProt Protein Name: LIM domain kinase 2
• Protein Family: LIM domain kinase
• UniProt Gene Name: LIMK2
• UniProt Synonym Gene Names: LIMK-2

NCBI Description

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.