Western Blot (WB)
(Western blot analysis of extracts from K562 cells, using IRS-1 (Phospho-Ser307) antibody.)
IRS-1 Antibody | anti-IRS-1 antibody
IRS-1 (Phospho-Ser307) Antibody
Gene Names
IRS1; HIRS-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
IRS-1; IRS-1 (Phospho-Ser307) Antibody; Insulin receptor substrate-1; IRS1; anti-IRS-1 antibody
Reactivity
Human, Mouse, Rat
Specificity
IRS-1 (Phospho-Ser307) antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 307.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
1242
Applicable Applications for anti-IRS-1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:10000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:10000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 307 (T-E-SP-I-T).
All Sites
Human: Ser307
Mouse: Ser302
Rat: Ser302
Mouse: Ser302
Rat: Ser302
Preparation and Storage
Store at-20 degree C for 1 year.
Western Blot (WB)
(Western blot analysis of extracts from K562 cells, using IRS-1 (Phospho-Ser307) antibody.)
Western Blot (WB)
(Western blot analysis of extracts from K562 cells, using IRS-1 (Phospho-Ser307) antibody.)
Testing Data
(P-peptide-+ Immunofluorescence analysis of COS-7 cells, using IRS-1 (Phospho-Ser307) antibody.)
Testing Data
(P-peptide-+ Immunofluorescence analysis of COS-7 cells, using IRS-1 (Phospho-Ser307) antibody.)
References
Araki E., Diabetes 42:1041-1054(1993). Nishiyama M., Biochem. Biophys. Res. Commun. 183:280-285(1992). The MGC Project Team; Genome Res. 14:2121-2127(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
131,591 Da
NCBI Official Full Name
insulin receptor substrate 1
NCBI Official Synonym Full Names
insulin receptor substrate 1
NCBI Official Symbol
IRS1
NCBI Official Synonym Symbols
HIRS-1
NCBI Protein Information
insulin receptor substrate 1
UniProt Protein Name
Insulin receptor substrate 1
UniProt Gene Name
IRS1
UniProt Synonym Gene Names
IRS-1
NCBI Description
This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]
Uniprot Description
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit ().
Research Articles on IRS-1
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Product Notes
The IRS-1 irs1 (Catalog #AAA8510171) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The IRS-1 (Phospho-Ser307) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IRS-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000. Researchers should empirically determine the suitability of the IRS-1 irs1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IRS-1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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