Testing Data
(P-peptide-+)
HSL Antibody | anti-HSL antibody
HSL (Phospho-Ser552) Antibody
Gene Names
LIPE; HSL; LHS; AOMS4; FPLD6
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
HSL; HSL (Phospho-Ser552) Antibody; EC 3.1.1.79; Hormone sensitive lipase; LIPE; LIPS; anti-HSL antibody
Reactivity
Human, Mouse, Rat
Specificity
HSL (Phospho-Ser552) antibody detects endogenous levels of HSL only when phosphorylated at serine 552.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
1076
Applicable Applications for anti-HSL antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:5000
IHC: 1:50~1:100
ELISA: 1:5000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human HSL around the phosphorylation site of serine 552 (R-R-SP-V-S).
All Sites
Human: Ser552
Mouse: Ser557
Rat: Ser563
Mouse: Ser557
Rat: Ser563
Preparation and Storage
Store at-20 degree C for 1 year.
Testing Data
(P-peptide-+)
Testing Data
(P-peptide-+)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using HSL (Phospho-Ser552) antibody. Western blot analysis of extracts from K562 cells, using using HSL (Phospho-Ser552) antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using HSL (Phospho-Ser552) antibody. Western blot analysis of extracts from K562 cells, using using HSL (Phospho-Ser552) antibody.)
Product Categories/Family for anti-HSL antibody
References
Matthew J. Watt, Am J Physiol Endocrinol Metab, Mar 2006; 290: E500-E508. Kenji Harada, Am J Physiol Endocrinol Metab, Dec 2003; 285: E1182-E1195. Shu Pei Wang, Endocrinology, Dec 2004; 145: 5688-5693. Mélanie Fortier, J. Lipid Res., Sep 2005; 46: 1860-1867.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84,128 Da
NCBI Official Full Name
hormone-sensitive lipase
NCBI Official Synonym Full Names
lipase E, hormone sensitive type
NCBI Official Symbol
LIPE
NCBI Official Synonym Symbols
HSL; LHS; AOMS4; FPLD6
NCBI Protein Information
hormone-sensitive lipase
UniProt Protein Name
Hormone-sensitive lipase
UniProt Gene Name
LIPE
UniProt Synonym Gene Names
HSL
NCBI Description
The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008]
Uniprot Description
In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
Research Articles on HSL
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Product Notes
The HSL lipe (Catalog #AAA859743) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The HSL (Phospho-Ser552) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HSL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000. Researchers should empirically determine the suitability of the HSL lipe for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSL, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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