HMGB2 Antibody | anti-HMGB2 antibody

HMGB2 Antibody

Cat. Num. AAA8513871

• Gene Names: HMGB2; HMG2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Immunogen affinity purified
• Synonyms: HMGB2; HMGB2 Antibody; HMG2; High mobility group protein 2; anti-HMGB2 antibody

• Reactivity: Human, Mouse, Rat
• Specificity: HMGB2 Antibody detects endogenous levels of total HMGB2
• Purity/Purification: Immunogen affinity purified
• Form/Format: In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 209

• Applicable Applications for anti-HMGB2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthesized peptide
• Preparation and Storage: Store at-20 degree C for 1 year

Western Blot (WB)

(Western blot analysis of extracts from COLO205 cells, using HMGB2 antibody)

NCBI and Uniprot Product Information

• NCBI GI #: 194688133
• NCBI GeneID: 3148
• NCBI Accession #: NP_001124160.1
• NCBI GenBank Nucleotide #: NM_001130688.1
• UniProt Accession #: P26583; Q5U072; B2R4K8; D3DP37
• Molecular Weight: 27 KD
• NCBI Official Full Name: high mobility group protein B2
• NCBI Official Synonym Full Names: high mobility group box 2
• NCBI Official Symbol: HMGB2
• NCBI Official Synonym Symbols: HMG2
• NCBI Protein Information: high mobility group protein B2
• UniProt Protein Name: High mobility group protein B2
• Protein Family: High mobility group protein
• UniProt Gene Name: HMGB2
• UniProt Synonym Gene Names: HMG2; HMG-2

NCBI Description

This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. [provided by RefSeq, Jul 2008]

Uniprot Description

Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. In the nucleus is an abundant chromatin-associated non-histone protein involved in transcription, chromatin remodeling and V(D)J recombination and probably other processes. Binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters by enhancing transcription factor binding and/or bringing distant regulatory sequences into close proximity (PubMed:7797075, PubMed:11909973, PubMed:19522541, PubMed:18413230, PubMed:19965638, PubMed:20123072). Involved in V(D)J recombination by acting as a cofactor of the RAG complex: acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS) (). Proposed to be involved in the innate immune response to nucleic acids by acting as a promiscuous immunogenic DNA/RNA sensor which cooperates with subsequent discriminative sensing by specific pattern recognition receptors (). In the extracellular compartment acts as a chemokine. Promotes proliferation and migration of endothelial cells implicating AGER/RAGE (PubMed:19811285). Has antimicrobial activity in gastrointestinal epithelial tissues (PubMed:23877675). Involved in inflammatory response to antigenic stimulus coupled with proinflammatory activity (). Involved in modulation of neurogenesis probably by regulation of neural stem proliferation (). Involved in articular cartilage surface maintenance implicating LEF1 and the Wnt/beta-catenin pathway ().