Testing Data
(P-peptide-+)
HMG14 Antibody | anti-HMG14 antibody
HMG14 (Phospho-Ser21) Antibody
Gene Names
HMGN1; HMG14
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
HMG14; HMG14 (Phospho-Ser21) Antibody; High-mobility group nucleosome binding domain 1; Nonhistone chromosomal protein HMG-14; anti-HMG14 antibody
Reactivity
Human, Mouse, Rat
Specificity
HMG14 (Phospho-Ser21) antibody detects endogenous levels of HMG14 only when phosphorylated at serine 21.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
100
Applicable Applications for anti-HMG14 antibody
Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:1000
IF: 1:100-1:500
ELISA: 1:1000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human HMG14 around the phosphorylation site of serine 21 (R-R-SP-A-R).
All Sites
Human: Ser21
Mouse: Ser20
Rat: Ser21
Mouse: Ser20
Rat: Ser21
Preparation and Storage
Store at-20 degree C for 1 year.
Testing Data
(P-peptide-+)
Testing Data
(P-peptide-+)
Immunohistochemistry (IHC)
(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using HMG14 (Phospho-Ser21) antibody. Immunofluorescence analysis of COS7 cells, using HMG14 (Phospho-Ser21) antibody.)
Immunohistochemistry (IHC)
(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using HMG14 (Phospho-Ser21) antibody. Immunofluorescence analysis of COS7 cells, using HMG14 (Phospho-Ser21) antibody.)
Testing Data
(HMG14 (Phospho-Ser21) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
Testing Data
(HMG14 (Phospho-Ser21) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
Product Categories/Family for anti-HMG14 antibody
References
Mihael H. Polymeropoulos. Nucleic Acids Res., Jul 1991; 19: 3753. LJ Arwood. J. Biol. Chem., Jun 1990; 265: 9771-9777. Thomas Hildmann. Genome Res., Apr 1999; 9: 360-372.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,659 Da
NCBI Official Full Name
non-histone chromosomal protein HMG-14
NCBI Official Synonym Full Names
high mobility group nucleosome binding domain 1
NCBI Official Symbol
HMGN1
NCBI Official Synonym Symbols
HMG14
NCBI Protein Information
non-histone chromosomal protein HMG-14
UniProt Protein Name
Non-histone chromosomal protein HMG-14
Protein Family
UniProt Gene Name
HMGN1
UniProt Synonym Gene Names
HMG14
NCBI Description
The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMG17, the encoded protein may help maintain an open chromatin configuration around transcribable genes. [provided by RefSeq, Aug 2011]
Uniprot Description
Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2 ().
Research Articles on HMG14
Similar Products
Product Notes
The HMG14 hmgn1 (Catalog #AAA858437) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The HMG14 (Phospho-Ser21) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HMG14 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). IHC: 1:50-1:100 IF: 1:100-1:500 ELISA: 1:1000. Researchers should empirically determine the suitability of the HMG14 hmgn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HMG14, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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