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Immunohistochemistry (IHC) (Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GATA-1 (Ab-142) antibody.)

GATA-1 Antibody | anti-GATA-1 antibody

GATA-1 (Ab-142) Antibody

Gene Names
GATA1; GF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA, Immunoprecipitation
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
GATA-1; GATA-1 (Ab-142) Antibody; anti-GATA-1 antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse, Rat
Specificity
GATA-1 (Ab-142) antibody detects endogenous levels of total GATA-1 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
413
Applicable Applications for anti-GATA-1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA), Immunoprecipitation (IP)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:10000
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human GATA-1 around the phosphorylation site of serine 142.
Preparation and Storage
Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GATA-1 (Ab-142) antibody.)

Immunohistochemistry (IHC) (Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GATA-1 (Ab-142) antibody.)

Western Blot (WB)

(Western blot analysis of extracts from K562 cells using GATA-1 (Ab-142) antibody.)

Western Blot (WB) (Western blot analysis of extracts from K562 cells using GATA-1 (Ab-142) antibody.)
References
Zon LI, et al. (1990) Proc Natl Acad. Sci USA. 87: 668-672. Trainor C D, et al. (1990) Nature. 343: 92-96. Nichols K E, et al. (2000) Nat Genet. 24: 266-270. Freson K, et al. (2001) Blood. 98: 85-92. Mehaffey M G, et al. (2001) Blood. 98: 2681-2688.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34,232 Da
NCBI Official Full Name
erythroid transcription factor
NCBI Official Synonym Full Names
GATA binding protein 1
NCBI Official Symbol
GATA1
NCBI Official Synonym Symbols
GF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1
NCBI Protein Information
erythroid transcription factor
UniProt Protein Name
Erythroid transcription factor
UniProt Gene Name
GATA1
UniProt Synonym Gene Names
ERYF1; GF1; GATA-1; GF-1

NCBI Description

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. [provided by RefSeq, Jul 2008]

Uniprot Description

Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed:24245781).

Research Articles on GATA-1

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Product Notes

The GATA-1 gata1 (Catalog #AAA8510141) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The GATA-1 (Ab-142) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GATA-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000. Researchers should empirically determine the suitability of the GATA-1 gata1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GATA-1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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