Testing Data
(Image: (left) Adult rat brain hippocampus section (45 muM; fixed by transcardial perfusion with 4% paraformaldehyde) was stained (1:1,000, red), and our rabbit anti-MeCP2 (green). Calretinin labels a subset of hippocampal interneurons, which also express MeCP2 in the nucleus to give a yellow color. (Right) Adult rat cortex section was co-stained with Calretinin (red) and our mouse anti-calbindin antibody green). Each antibody specifically labels a subset of interneurons (i.e., calretinin-positive or calbindin-postive) that express each marker exclusively. Insets are high-magnification images of the boxed area in each picture. Blue is Dapi staining that labels DNA.)
Calretinin Antibody | anti-CALB2 antibody
Calretinin, chicken IgY
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Synonyms
Calretinin; chicken IgY; anti-CALB2 antibody
Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Sequence Length
87
Applicable Applications for anti-CALB2 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Testing Data
(Image: (left) Adult rat brain hippocampus section (45 muM; fixed by transcardial perfusion with 4% paraformaldehyde) was stained (1:1,000, red), and our rabbit anti-MeCP2 (green). Calretinin labels a subset of hippocampal interneurons, which also express MeCP2 in the nucleus to give a yellow color. (Right) Adult rat cortex section was co-stained with Calretinin (red) and our mouse anti-calbindin antibody green). Each antibody specifically labels a subset of interneurons (i.e., calretinin-positive or calbindin-postive) that express each marker exclusively. Insets are high-magnification images of the boxed area in each picture. Blue is Dapi staining that labels DNA.)
Testing Data
(Image: (left) Adult rat brain hippocampus section (45 muM; fixed by transcardial perfusion with 4% paraformaldehyde) was stained (1:1,000, red), and our rabbit anti-MeCP2 (green). Calretinin labels a subset of hippocampal interneurons, which also express MeCP2 in the nucleus to give a yellow color. (Right) Adult rat cortex section was co-stained with Calretinin (red) and our mouse anti-calbindin antibody green). Each antibody specifically labels a subset of interneurons (i.e., calretinin-positive or calbindin-postive) that express each marker exclusively. Insets are high-magnification images of the boxed area in each picture. Blue is Dapi staining that labels DNA.)
Related Product Information for anti-CALB2 antibody
Calretinin is a member of the large superfamily of cytoplasmic Ca2+ binding proteins, thus belongs to the subclass of these containing the "EF hand" Ca2+ binding motif originally characterized in parvalbumin. Calretinin is expressed in mammalian central nerve system, testis, fallopian tube and pancreas. In the brain it is localized in certain classes of neurons, and antibodies to it are useful for identifying specific neuronal cell types. It is particularly concentrated in some cerebellar granular cells and their parallel fibers, but is also found in many GABAergic interneurons in the cortex. These GABAergic interneurons, in most cases, express only one of three Ca2+ binding proteins, namely calretinin, calbindin or parvalbumin. As a result, these important inhibitory interneurons can be identified and subclassified based on their content of these three proteins. Each type of neuron as defined in this fashion has particular electrophysiological and functional properties. For example, calbindin positive interneurons are not fast-spiking as are parvalbumin expressinginterneurons. Human calretinin is also known as 29 kDa calbindin and calbindin-2, for its sequence is related to calbindin.
Calretinin contains six EF-hand domains. Four of them bind Ca2+ with high affinity in a cooperative manner, one with low affinity and the last one is non-functional, without Ca2+-binding ability. The function of calretinin appears to be primarily buffering the Ca2+ level in cells and affect intracellular calcium signals. Calretinin deficiency in mossy cells of the dentate gyrus and granule cells results in abnormal excitability in the cerebellar neuronal network and impairment of long-term potentiation and motor coordination. The HGNC name for this protein is CALB2.
This antibody was raised against human calretinin protein expressed in and purified from E Coli. This antibody does not cross-react with the related calcium binding proteins calbindin and parvalbumin (see Blot image).
Calretinin contains six EF-hand domains. Four of them bind Ca2+ with high affinity in a cooperative manner, one with low affinity and the last one is non-functional, without Ca2+-binding ability. The function of calretinin appears to be primarily buffering the Ca2+ level in cells and affect intracellular calcium signals. Calretinin deficiency in mossy cells of the dentate gyrus and granule cells results in abnormal excitability in the cerebellar neuronal network and impairment of long-term potentiation and motor coordination. The HGNC name for this protein is CALB2.
This antibody was raised against human calretinin protein expressed in and purified from E Coli. This antibody does not cross-react with the related calcium binding proteins calbindin and parvalbumin (see Blot image).
NCBI and Uniprot Product Information
Uniprot Description
CALB2: Calretinin is a calcium-binding protein which is abundant in auditory neurons. Belongs to the calbindin family.
Protein type: Calcium-binding
Chromosomal Location of Human Ortholog: 16q22.2
Cellular Component: cytosol; neuron projection; nucleus; synapse
Molecular Function: calcium ion binding
Biological Process: cytosolic calcium ion homeostasis
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Product Notes
The Calretinin calb2 (Catalog #AAA555647) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Calretinin, chicken IgY reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Calretinin can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the Calretinin calb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calretinin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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