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Testing Data (Peptide-+)

BID Antibody | anti-BID antibody

BID (Ab-78) Antibody

Gene Names
BID; FP497
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
BID; BID (Ab-78) Antibody; BH3 interacting domain death agonist; anti-BID antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse, Rat
Specificity
BID (Ab-78) antibody detects endogenous levels of total BID protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
195
Applicable Applications for anti-BID antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:10000
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human BID around the phosphorylation site of serine 78(S-E-SP-Q-E).
Preparation and Storage
Store at-20 degree C for 1 year.

Testing Data

(Peptide-+)

Testing Data (Peptide-+)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human brain tissue using BID (Ab-78) antibody. Western blot analysis of extracts from Jurkat cells treated with H2O2 (100uM, 30mins) (lane1) and Jurkat cells treated with Etoposide (25uM, 60mins) (lane2), using BID (Ab-78) antibody.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using BID (Ab-78) antibody. Western blot analysis of extracts from Jurkat cells treated with H2O2 (100uM, 30mins) (lane1) and Jurkat cells treated with Etoposide (25uM, 60mins) (lane2), using BID (Ab-78) antibody.)
References
Wang K., Genes Dev. 10:2859-2869(1996). Footz T.K., Genomics 51:472-475(1998). Renshaw S.A., J. Biol. Chem. 279:2846-2855(2004).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
637
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
11,263 Da
NCBI Official Full Name
BH3-interacting domain death agonist isoform 2
NCBI Official Synonym Full Names
BH3 interacting domain death agonist
NCBI Official Symbol
BID
NCBI Official Synonym Symbols
FP497
NCBI Protein Information
BH3-interacting domain death agonist
UniProt Protein Name
BH3-interacting domain death agonist
UniProt Gene Name
BID
UniProt Synonym Gene Names
BID

NCBI Description

This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]

Uniprot Description

The major proteolytic product p15 BID allows the release of cytochrome c (). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.

Research Articles on BID

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Product Notes

The BID bid (Catalog #AAA8500793) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The BID (Ab-78) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BID can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000. Researchers should empirically determine the suitability of the BID bid for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BID, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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