Western Blot (WB)
(Western blot analysis of extracts from RAW264.7 cells, using ARHGEF12 antibody.)
ARHGEF12 Antibody | anti-ARHGEF12 antibody
ARHGEF12 Antibody
Gene Names
ARHGEF12; LARG; PRO2792
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
ARHGEF12; ARHGEF12 Antibody; ARHGC; Leukemia-associated RhoGEF; RHO guanine nucleotide exchange factor (GEF) 12 (LARG); Rho guanine nucleotide exchange factor 12; anti-ARHGEF12 antibody
Reactivity
Human, Mouse, Rat
Specificity
ARHGEF12 antibody detects endogenous levels of total ARHGEF12 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
1525
Applicable Applications for anti-ARHGEF12 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:20000
ELISA: 1:20000
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human ARHGEF12.
Preparation and Storage
Store at-20 degree C for 1 year.
Western Blot (WB)
(Western blot analysis of extracts from RAW264.7 cells, using ARHGEF12 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from RAW264.7 cells, using ARHGEF12 antibody.)
Product Categories/Family for anti-ARHGEF12 antibody
References
Kourlas P.J., Proc. Natl. Acad. Sci. U.S.A. 97:2145-2150(2000). The MGC Project Team, Genome Res. 14:2121-2127(2004). Nagase T., DNA Res. 4:141-150(1997).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
171,115 Da
NCBI Official Full Name
rho guanine nucleotide exchange factor 12 isoform 2
NCBI Official Synonym Full Names
Rho guanine nucleotide exchange factor 12
NCBI Official Symbol
ARHGEF12
NCBI Official Synonym Symbols
LARG; PRO2792
NCBI Protein Information
rho guanine nucleotide exchange factor 12
UniProt Protein Name
Rho guanine nucleotide exchange factor 12
Protein Family
UniProt Gene Name
ARHGEF12
UniProt Synonym Gene Names
KIAA0382; LARG
NCBI Description
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
Uniprot Description
May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13.
Research Articles on ARHGEF12
Similar Products
Product Notes
The ARHGEF12 arhgef12 (Catalog #AAA855284) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The ARHGEF12 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ARHGEF12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:20000. Researchers should empirically determine the suitability of the ARHGEF12 arhgef12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARHGEF12, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
