iNOS Phospho-Regulation Antibody Sampler Kit | iNOS antibody sampler kit
iNOS Phospho-Regulation
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA, Immunocytochemistry
Synonyms
iNOS Phospho-Regulation; iNOS antibody sampler kit
Reactivity
Human, Rat, Mouse
Form/Format
Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide.
Applicable Applications for iNOS antibody sampler kit
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
Preparation and Storage
Store all at -20 degree C.Stable for 1 year.
Related Product Information for iNOS antibody sampler kit
Kit Summary: The inducible nitric oxide synthase (iNOS) phospho-regulation antibody sampler kit can be used to examine Tyr-1055 phosphorylation in iNOS. The kit also includes a mouse monoclonal and rabbit polyclonal antibody to monitor totalexpression levels of iNOS, as well as HRP-conjugated secondary reagents.
Background: Nitric oxide (NO) has a broad range of biological activities and is implicated in many cell signaling pathways. Nitric oxidesynthases (NOS), the enzymes responsible for synthesis of NO, are fused enzymes that include a cytochrome reductaseand a cytochrome that requires three cosubstrates (L-arginine, NADPH, and oxygen) and five cofactors (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). The NOS family includes two constitutive Ca2+/CaM-dependent forms ofNOS: nNOS (also designated bNOS, NOS-I), whose activity was first identified in neurons and eNOS (also designatedecNOS, NOS-III) first identified in endothelial cells. The inducible form of NOS, iNOS (also designated NOS-II), is Ca2+independent and is induced by microbial products in various cell types. Phosphorylation of NOS enzymes may regualtetheir activity. For example, Src kinase-induced phosphorylation of Tyr-1055 in iNOS reduces proteasomal degradation ofiNOS, while shear stress induced phosphorylation of Tyr-657 in eNOS attenuates enzyme activity. Both of these tyrosinesites are conserved in nNOS, and may have important roles for regulate neuronal NOS activity.
Background: Nitric oxide (NO) has a broad range of biological activities and is implicated in many cell signaling pathways. Nitric oxidesynthases (NOS), the enzymes responsible for synthesis of NO, are fused enzymes that include a cytochrome reductaseand a cytochrome that requires three cosubstrates (L-arginine, NADPH, and oxygen) and five cofactors (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). The NOS family includes two constitutive Ca2+/CaM-dependent forms ofNOS: nNOS (also designated bNOS, NOS-I), whose activity was first identified in neurons and eNOS (also designatedecNOS, NOS-III) first identified in endothelial cells. The inducible form of NOS, iNOS (also designated NOS-II), is Ca2+independent and is induced by microbial products in various cell types. Phosphorylation of NOS enzymes may regualtetheir activity. For example, Src kinase-induced phosphorylation of Tyr-1055 in iNOS reduces proteasomal degradation ofiNOS, while shear stress induced phosphorylation of Tyr-657 in eNOS attenuates enzyme activity. Both of these tyrosinesites are conserved in nNOS, and may have important roles for regulate neuronal NOS activity.
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Product Notes
The Human, Rat, Mouse iNOS (Catalog #AAA474464) is an Antibody Sampler Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA474464 Antibody Sampler Kit recognizes Human, Rat, Mouse iNOS. AAA Biotech's iNOS Phospho-Regulation can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the iNOS for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "iNOS Phospho-Regulation, Antibody Sampler Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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