Histone H2B Phospho-Regulation Antibody Sampler Kit
Histone H2B Phospho-Regulation
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA
Synonyms
Histone H2B Phospho-Regulation; Histone H2BK; Histone H2B Phospho-Regulation antibody sampler kit
Reactivity
Human, Rat, Mouse
Form/Format
Rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide.
Applicable Applications for Histone H2B Phospho-Regulation antibody sampler kit
Western Blot (WB), ELISA (EIA)
Preparation and Storage
Store all at -20 degree C. Stable for 1 year.
Related Product Information for Histone H2B Phospho-Regulation antibody sampler kit
Kit Summary: The histone H2B phospho-regulation kit can be used to examine phosphorylation of Ser-36 compared to the totalexpression of Histone H2B. The kit includes two different rabbit polyconal antibodies to detect total Histone H2B, andsecondary reagent for rabbit polyclonal antibody detection.
Background: The nucleosome is a protein complex consisting of four core histones (H2A, H2B, H3, and H4). Two molecules of eachhistone forms an octamer that makes up the nucleosome. DNA wraps around repeating nucleosome units to generatechromatin structures. The structure of chromatin determines the accessiblity to transcription factors. Post-translationalmodification of the amino-terminal tail of histones in nucleosomes alters chromatin structure to promote or inhibittranscription. Complex alterations in acetylation, methylation, ubiquination, and/or phosphorylation determine thechromatin structural changes that occur during specific phases of the cell cycle or in response to cell stimuli. One mode ofregulating histone H2B activity is through phosphorylation in the amino terminal region. Important sites of phosphorylationinclude Ser-14, Ser-32, and Ser-36. AMPK phosphorylates Ser-36 on histone H2B during cell stress leading to increasedtranscription and cell survival, while ectopic expression of an unphosphorylatable histone H2B during cell stress reducestranscription of AMPK-dependent genes and lowers cell survival.
Background: The nucleosome is a protein complex consisting of four core histones (H2A, H2B, H3, and H4). Two molecules of eachhistone forms an octamer that makes up the nucleosome. DNA wraps around repeating nucleosome units to generatechromatin structures. The structure of chromatin determines the accessiblity to transcription factors. Post-translationalmodification of the amino-terminal tail of histones in nucleosomes alters chromatin structure to promote or inhibittranscription. Complex alterations in acetylation, methylation, ubiquination, and/or phosphorylation determine thechromatin structural changes that occur during specific phases of the cell cycle or in response to cell stimuli. One mode ofregulating histone H2B activity is through phosphorylation in the amino terminal region. Important sites of phosphorylationinclude Ser-14, Ser-32, and Ser-36. AMPK phosphorylates Ser-36 on histone H2B during cell stress leading to increasedtranscription and cell survival, while ectopic expression of an unphosphorylatable histone H2B during cell stress reducestranscription of AMPK-dependent genes and lowers cell survival.
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Product Notes
The Human, Rat, Mouse Histone H2B Phospho-Regulation (Catalog #AAA474374) is an Antibody Sampler Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA474374 Antibody Sampler Kit recognizes Human, Rat, Mouse Histone H2B Phospho-Regulation. AAA Biotech's Histone H2B Phospho-Regulation can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Researchers should empirically determine the suitability of the Histone H2B Phospho-Regulation for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2B Phospho-Regulation, Antibody Sampler Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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