Bad Phospho-Regulation Antibody Sampler Kit | Bad antibody sampler kit
Bad Phospho-Regulation
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA, Immunocytochemistry, Immunohistochemistry
Synonyms
Bad Phospho-Regulation; Bad antibody sampler kit
Reactivity
Human, Rat, Mouse
Form/Format
Primary antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. BP4971 is supplied in phosphate-buffered saline and0.09% sodium azide.
Applicable Applications for Bad antibody sampler kit
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
Preparation and Storage
Store all at -20 degree C. Stable for 1 year.
Related Product Information for Bad antibody sampler kit
Kit Summary: The Bad phospho-regulation antibody sampler kit can be used to detect phosphorylation of Ser-26 and Ser-112 relative tototal Bad expression levels. The kit includes rabbit polyclonal phospho-specific antibodies to Ser-26 and Ser-112, as wellas a mouse monoclonal antibody for detection of total Bad. The kit also includes anti-Rabbit Light Chain specific:HRP andanti-Mouse Ig:HRP secondary reagents for detection of antibodies in western blot, ELISA, or immunocytochemistry.
Background: Bad is a member of the BCL-2 family of regulators involved in programmed cell death. This protein positively regulatescell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptoticactivity of this protein is regulated through its phosphorylation. Protein kinases AKT IKK, and MAP kinases, as well asprotein phosphatase calcineurin are found to be involved in the regulation of this Bad activity. Phosphorylation of Badoccurs on one or more of Ser-26, Ser-112, Ser-136, and Ser-155 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-136 or Ser-112 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-155, a site within the BH3 motif, leading to the release of Bcl-xL andthe promotion of cell survival. Ser-26 is phosphorylated by IKK leading to phosphorylation of C-terminal serine sites and disruption of binding to Bcl-xL. This inactivation of Bad inhibits TNFalpha-induced apoptosis independent of NF-kappaB activity.
Background: Bad is a member of the BCL-2 family of regulators involved in programmed cell death. This protein positively regulatescell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptoticactivity of this protein is regulated through its phosphorylation. Protein kinases AKT IKK, and MAP kinases, as well asprotein phosphatase calcineurin are found to be involved in the regulation of this Bad activity. Phosphorylation of Badoccurs on one or more of Ser-26, Ser-112, Ser-136, and Ser-155 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-136 or Ser-112 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-155, a site within the BH3 motif, leading to the release of Bcl-xL andthe promotion of cell survival. Ser-26 is phosphorylated by IKK leading to phosphorylation of C-terminal serine sites and disruption of binding to Bcl-xL. This inactivation of Bad inhibits TNFalpha-induced apoptosis independent of NF-kappaB activity.
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Product Notes
The Human, Rat, Mouse Bad (Catalog #AAA474196) is an Antibody Sampler Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA474196 Antibody Sampler Kit recognizes Human, Rat, Mouse Bad. AAA Biotech's Bad Phospho-Regulation can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the Bad for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bad Phospho-Regulation, Antibody Sampler Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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