TRACP5a elisa kit
TRACP5a, Human, ELISA kit
Does not react: Mouse, Pig, Rabbit, Rat
Does not react: Mouse, Pig, Rabbit, Rat
Under recommended storage conditions, product is stable for at least six months.
Background/Introduction: Tartrate-resistant acid phosphatase (TRACP) is a biomarker with multiple clinical and basic applications. It has long been used as a cytochemical marker to identify hairy cell leukemia cells and as a marker for differentiated cells of monocyte lineage including macrophages (Mphi), osteoclasts (OC), and dendritic cells (DC). The clinically relevant Mphi/OC/DC TRACP is called Type-5 acid phosphatase encoded by the Acp5 gene. TRACP consists of two isoforms (Type 5a and 5b) that have different biochemical properties, clinical significance and perhaps biological functions. TRACP5a circulates as a 35 kDa glycoprotein with an intact central regulatory loop peptide, which interacts with the active site. TRACP5b circulates as a disulfide linked heterodimer in which the loop peptide has been proteolytically processed to yield 16 kDa and 23 kDa fragments. Osteoclasts secrete TRACP5b during bone resorption making it a sensitive and specific serum biomarker for OC number and bone resorption rate. TRACP5a, but not 5b, is secreted by activated macrophages and dendritic cells. Elevated serum TRACP5a is emerging as a potential marker for systemic macrophages and chronic inflammation. The principal clinical application of serum TRACP immunoassay is to estimate isoform 5b activity as a marker of osteoclast number and bone resorption rate. This is done in commercial immunoassays by increasing the pH to a level selectively permissive for isoform 5b activity and / or by using a substrate selective for isoform 5b. The antibody used it this ELISA reacts with a critical epitope and binding to TRACP protein causes inactivation of the enzymatic activity.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq, Aug 2008]
Uniprot Description
ACP5: Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. Defects in ACP5 are the cause of spondyloenchondrodysplasia with immune dysregulation (SPENCDI). A disease characterized by vertebral and metaphyseal dysplasia, spasticity with cerebral calcifications, and strong predisposition to autoimmune diseases. The skeletal dysplasia is characterized by radiolucent and irregular spondylar and metaphyseal lesions that represent islands of chondroid tissue within bone. ACP5 inactivating mutations result in a functional excess of phosphorylated osteopontin causing deregulation of osteopontin signaling and consequential autoimmune disease. Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.
Protein type: Motility/polarity/chemotaxis; Phosphatase; EC 3.1.3.2; Cofactor and Vitamin Metabolism - riboflavin
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: lysosome; integral to membrane; cytosol
Molecular Function: acid phosphatase activity; ferric iron binding; ferrous iron binding
Biological Process: negative regulation of interleukin-12 production; vitamin metabolic process; negative regulation of nitric oxide biosynthetic process; response to lipopolysaccharide; negative regulation of interleukin-1 beta production; negative regulation of superoxide release; negative regulation of tumor necrosis factor production; riboflavin metabolic process; defense response to Gram-positive bacterium; dephosphorylation; response to cytokine stimulus; negative regulation of inflammatory response; water-soluble vitamin metabolic process; bone resorption
Disease: Spondyloenchondrodysplasia With Immune Dysregulation
Research Articles on TRACP5a
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Product Notes
The Human Does not react: Mouse, Pig, Rabbit, Rat TRACP5a acp5 (Catalog #AAA584840) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA584840 ELISA Kit recognizes Human Does not react: Mouse, Pig, Rabbit, Rat TRACP5a. It is sometimes possible for the material contained within the vial of "TRACP5a, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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