Human, Mouse SEPARASE ELISA Kit | SEPARASE elisa kit
Phospho-SEPARASE (Ser801) Colorimetric Cell-Based ELISA Kit
NCBI and Uniprot Product Information
NCBI Description
Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM, Nov 2010]
Uniprot Description
separase: a caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms. Regulated by at least two independent mechanisms. First, it is inactivated via its interaction with securin/PTTG1, which probably covers its active site. Second, phosphorylation inactivates it. It is completely phosphorylated during mitosis and dephosphorylated during anaphase. Two alternatively spliced isoforms have been described.
Protein type: EC 3.4.22.49; Protease
Chromosomal Location of Human Ortholog: 12q13.13
Cellular Component: centrosome; cytoplasm; cytosol; nucleus
Molecular Function: catalytic activity; cysteine-type endopeptidase activity; cysteine-type peptidase activity; protein binding
Biological Process: apoptosis; cytokinesis; establishment of mitotic spindle localization; homologous chromosome segregation; meiotic chromosome separation; meiotic spindle organization; mitotic sister chromatid segregation; negative regulation of sister chromatid cohesion; positive regulation of mitotic metaphase/anaphase transition; proteolysis
Research Articles on SEPARASE
Similar Products
Product Notes
The Human, Mouse SEPARASE espl1 (Catalog #AAA9501035) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9501035 ELISA Kit recognizes Human, Mouse SEPARASE. It is sometimes possible for the material contained within the vial of "SEPARASE, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
