Human, Mouse AF10 ELISA Kit | AF10 elisa kit

AF10 Colorimetric Cell-Based ELISA Kit

Cat. Num. AAA9500784

• Gene Names: MLLT10; AF10
• Reactivity: Human, Mouse
• Synonyms: AF10; AF10 Colorimetric Cell-Based ELISA Kit; Protein AF-10; MLLT10; AF10 elisa kit

• Reactivity: Human, Mouse
• Form/Format: One 96-Well Plate
• Sequence Length: 1068

• Preparation and Storage: Store at 4 degree C.

Related Product Information for AF10 elisa kit

Background/Introduction: The Colorimetric Cell-Based ELISA Kit allows for the detection of various target proteins and the effects that certain stimulation conditions have on target protein expression in different cell lines. Qualitative determination of target protein concentration is achieved by an indirect ELISA format. In essence, the target protein is captured by target-specific primary (1 degree) antibodies while the HRP-conjugated secondary (2 degree) antibodies bind the Fc region of the 1 degree antibody. Through this binding, the HRP enzyme conjugated to the 2 degree antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell- Based ELISA, multiple normalization methods are described: 1) a monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) If a phosphorylated target is being detected, an antibody against the non-phosphorylated counterpart will be provided for normalization purposes. The absorbance values obtained for the nonphosphorylated target can be used to normalize the absorbance values for the phosphorylated target.

Product Categories/Family for AF10 elisa kit

Cell Based ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 307548832
• NCBI GeneID: 8028
• NCBI Accession #: NP_001182555.1
• NCBI GenBank Nucleotide #: NM_001195626.1
• UniProt Accession #: P55197; Q5JT37; Q5VX90; Q66K63; B1ANA8
• Molecular Weight: 109 kDa
• NCBI Official Full Name: protein AF-10 isoform c
• NCBI Official Synonym Full Names: MLLT10, histone lysine methyltransferase DOT1L cofactor
• NCBI Official Symbol: MLLT10
• NCBI Official Synonym Symbols: AF10
• NCBI Protein Information: protein AF-10
• UniProt Protein Name: Protein AF-10
• UniProt Gene Name: MLLT10
• UniProt Synonym Gene Names: AF10

NCBI Description

This gene encodes a transcription factor and has been identified as a partner gene involved in several chromosomal rearrangements resulting in various leukemias. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]

Uniprot Description

MLLT10: Probably involved in transcriptional regulation. In vitro or as fusion protein with MLL has transactivation activity. Binds to cruciform DNA. A chromosomal aberration involving MLLT10 is associated with acute leukemias. Translocation t(10;11)(p12;q23) with MLL/HRX. The result is a rogue activator protein. A chromosomal aberration involving MLLT10 is associated with diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with PICALM. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 10p12.31

Cellular Component: cytosol; nucleoplasm; nucleus; protein complex

Molecular Function: DNA binding; DNA binding transcription factor activity; metal ion binding; protein binding

Biological Process: positive regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent

Disease: Leukemia, Acute Myeloid