anti-Human, Mouse USP13 Antibody | anti-USP13 antibody

USP13 Antibody

Cat. Num. AAA851394

• Gene Names: USP13; ISOT3; IsoT-3
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: USP13; USP13 Antibody; Ubiquitin carboxyl-terminal hydrolase 13; EC 3.1.2.15; Ubiquitin thioesterase 13; Ubiquitin-specific-processing protease 13; Deubiquitinating enzyme 13; Isopeptidase T-3; ISOT-3; ISOT3; anti-USP13 antibody

• Reactivity: Human, Mouse
• Specificity: USP13 antibody detects endogenous levels of total USP13 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 863

• Applicable Applications for anti-USP13 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:40000
• Immunogen: The antiserum was produced against synthesized peptide derived from internal of human USP13.
• Preparation and Storage: Store at-20 degree C for 1 year.

Testing Data #3

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using USP13 antibody.Western blot analysis of extracts from mouse brain cells, using USP13 antibody.)

Product Categories/Family for anti-USP13 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Timms K.M., Gene 217:101-106(1998). Mural R.J., Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Dephoure N., Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 215598688
• NCBI GeneID: 8975
• NCBI Accession #: NP_003931.2
• NCBI GenBank Nucleotide #: NM_003940.2
• UniProt Accession #: Q92995; Q96B25; A8K2S3; B4DYF3; D3DNS2
• Molecular Weight: 90,383 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 13
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 13
• NCBI Official Symbol: USP13
• NCBI Official Synonym Symbols: ISOT3; IsoT-3
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 13
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 13
• UniProt Gene Name: USP13
• UniProt Synonym Gene Names: ISOT3; ISOT-3

Uniprot Description

Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Also regulates ERAD through the deubiquitination of UBL4A a component of the BAG6/BAT3 complex. Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data.