Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western blot analysis of extracts from HeLa cells, Jurkat cells and 293 cells, using RAD54 antibody.)

anti-Human, Mouse RAD54 Antibody | anti-RAD54 antibody

RAD54 Antibody

Gene Names
RAD54L; HR54; hHR54; RAD54A; hRAD54
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
RAD54; RAD54 Antibody; Double-strand-break repair protein rad21 homolog; hHR21; Nuclear matrix protein 1; NXP-1; SCC1 homolog; RAD21; HR21; KIAA0078; NXP1; anti-RAD54 antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse
Specificity
RAD54 antibody detects endogenous levels of total RAD54 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
747
Applicable Applications for anti-RAD54 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:1000
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human RAD54.
Preparation and Storage
Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, Jurkat cells and 293 cells, using RAD54 antibody.)

Western Blot (WB) (Western blot analysis of extracts from HeLa cells, Jurkat cells and 293 cells, using RAD54 antibody.)
References
McKay M.J., Genomics 36:305-315(1996). Sadano H., Biochem. Biophys. Res. Commun. 267:418-422(2000). Nomura N., DNA Res. 1:223-229(1994).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
84,352 Da
NCBI Official Full Name
DNA repair and recombination protein RAD54-like
NCBI Official Synonym Full Names
RAD54 like
NCBI Official Symbol
RAD54L
NCBI Official Synonym Symbols
HR54; hHR54; RAD54A; hRAD54
NCBI Protein Information
DNA repair and recombination protein RAD54-like
UniProt Protein Name
DNA repair and recombination protein RAD54-like
UniProt Gene Name
RAD54L
UniProt Synonym Gene Names
RAD54A; hHR54; hRAD54

NCBI Description

The protein encoded by this gene belongs to the DEAD-like helicase superfamily, and shares similarity with Saccharomyces cerevisiae Rad54, a protein known to be involved in the homologous recombination and repair of DNA. This protein has been shown to play a role in homologous recombination related repair of DNA double-strand breaks. The binding of this protein to double-strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring, and stimulate DNA recombination. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Dec 2008]

Uniprot Description

Involved in DNA repair and mitotic recombination. Functions in the recombinational DNA repair (RAD52) pathway. Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments (). May play also an essential role in telomere length maintenance and telomere capping in mammalian cells.

Research Articles on RAD54

Similar Products

Product Notes

The RAD54 rad54l (Catalog #AAA852795) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The RAD54 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RAD54 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:1000. Researchers should empirically determine the suitability of the RAD54 rad54l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAD54, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.