anti-Human, Mouse NFAT2 Antibody | anti-NFAT2 antibody

NFAT2 (Phospho-Ser294) Antibody

Cat. Num. AAA858878

• Gene Names: NFATC1; NFAT2; NFATc; NF-ATC; NF-ATc1.2
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: NFAT2; NFAT2 (Phospho-Ser294) Antibody; NF-ATc; NF-ATc1; NFAC1; NFAT transcription complex cytosolic component; NFATC; NFATC1; NFC1; Nuclear factor of activated T-cells cytoplasmic 1; anti-NFAT2 antibody

• Reactivity: Human, Mouse
• Specificity: NFAT2 (Phospho-Ser294) antibody detects endogenous levels of NFAT2 only when phosphorylated at serine 294.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 943

• Applicable Applications for anti-NFAT2 antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human NFAT2 around the phosphorylation site of serine 294 (R-V-SP-V-T).
• All Sites: Human: Ser294; Mouse: Ser296
• Preparation and Storage: Store at-20 degree C for 1 year.

Immunohistochemistry (IHC)

(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human heart tissue using NFAT2 (Phospho-Ser294) antibody.)

Product Categories/Family for anti-NFAT2 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Gauci S., Anal. Chem. 81:4493-4501(2009). Northrop J.P., Nature 369:497-502(1994).

NCBI and Uniprot Product Information

• NCBI GI #: 522021835
• NCBI GeneID: 4772
• NCBI Accession #: NP_001265598.1
• NCBI GenBank Nucleotide #: NM_001278669.1
• UniProt Accession #: O95644; Q12865; Q15793; Q2M1S3; B5B2M4; B5B2M5; B5B2M6; B5B2M7; B5B2M8; B5B2M9; B5B2N1
• Molecular Weight: 38,626 Da
• NCBI Official Full Name: nuclear factor of activated T-cells, cytoplasmic 1 isoform F
• NCBI Official Synonym Full Names: nuclear factor of activated T cells 1
• NCBI Official Symbol: NFATC1
• NCBI Official Synonym Symbols: NFAT2; NFATc; NF-ATC; NF-ATc1.2
• NCBI Protein Information: nuclear factor of activated T-cells, cytoplasmic 1
• UniProt Protein Name: Nuclear factor of activated T-cells, cytoplasmic 1
• UniProt Gene Name: NFATC1
• UniProt Synonym Gene Names: NFAT2; NFATC; NF-ATc1; NFATc1; NF-ATc; NFATc

NCBI Description

The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq, Jul 2013]

Uniprot Description

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells (PubMed:10358178). Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function ().