anti-Human, Mouse MSK1 Antibody | anti-MSK1 antibody

MSK1 (Phospho-Ser360) Antibody

Cat. Num. AAA8509563

• Gene Names: RPS6KA5; MSK1; RLPK; MSPK1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: MSK1; MSK1 (Phospho-Ser360) Antibody; 90 kDa ribosomal protein S6 kinase; polypeptide 5; EC 2.7.11.1; KS6A5; MSPK1; Nuclear mitogen-and stress-activated protein kinase-1; RLPK; RPS6KA5; kinase MSK1; ribosomal protein S6 kinase; polypeptide V; anti-MSK1 antibody

• Reactivity: Human, Mouse
• Specificity: MSK1 (Phospho-Ser360) antibody detects endogenous levels of MSK1 only when phosphorylated at serine 360.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 723

• Applicable Applications for anti-MSK1 antibody: Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: IHC: 1:50-1:100; IF: 1:100-1:500; ELISA: 1:10000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human MSK1 around the phosphorylation site of serine 360 (T-Y-SP-P-A).
• All Sites: Human: Ser360; Mouse: Ser359
• Preparation and Storage: Store at-20 degree C for 1 year.

Testing Data

(P-peptide-+)

Immunohistochemistry (IHC)

(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MSK1 (Phospho-Ser360) antibody. Immunofluorescence analysis of NIH/3T3 cells, using MSK1 (Phospho-Ser360) antibody.)

References

Deak M., EMBO J. 17:4426-4441(1998). New L., J. Biol. Chem. 274:1026-1032(1999). Zhang Y., J. Biol. Chem. 279:21866-21872(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 1016080537
• NCBI GeneID: 9252
• NCBI Accession #: NP_001309164.1
• NCBI GenBank Nucleotide #: NM_001322235.1
• UniProt Accession #: O75582; O95316; Q96AF7; B7Z2Y5
• Molecular Weight: 81,780 Da
• NCBI Official Full Name: ribosomal protein S6 kinase alpha-5 isoform k
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase A5
• NCBI Official Symbol: RPS6KA5
• NCBI Official Synonym Symbols: MSK1; RLPK; MSPK1
• NCBI Protein Information: ribosomal protein S6 kinase alpha-5
• UniProt Protein Name: Ribosomal protein S6 kinase alpha-5
• UniProt Gene Name: RPS6KA5
• UniProt Synonym Gene Names: MSK1; S6K-alpha-5; RSKL

Uniprot Description

Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes (PubMed:11909979, PubMed:12569367, PubMed:12763138, PubMed:9687510, PubMed:18511904, PubMed:9873047). Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin (PubMed:11909979, PubMed:9873047). Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression (PubMed:12628924, PubMed:18511904). In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress (PubMed:12628924). In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential (PubMed:12763138). Phosphorylates ETV1/ER81 at 'Ser-191' and 'Ser-216', and thereby regulates its ability to stimulate transcription, which may be important during development and breast tumor formation (PubMed:12569367). Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A (PubMed:15010469). Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN (PubMed:12773393). May also phosphorylate 'Ser-28' of histone H3 (PubMed:12773393). Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14) (PubMed:12773393). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines (). Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors (). Plays a role in neuronal cell death by mediating the downstream effects of excitotoxic injury (). Phosphorylates TRIM7 at 'Ser-107' in response to growth factor signaling via the MEK/ERK pathway, thereby stimulating its ubiquitin ligase activity (PubMed:25851810).