Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western blot analysis of extracts from HeLa cells and HUVEC cells, using MRPS27 antibody.)

anti-Human, Mouse MRPS27 Antibody | anti-MRPS27 antibody

MRPS27 Antibody

Gene Names
MRPS27; S27mt; MRP-S27
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
MRPS27; MRPS27 Antibody; mitochondrial 28S ribosomal protein S27; mitochondrial ribosomal protein S27; MRP-S27; RT27; S27mt; anti-MRPS27 antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse
Specificity
MRPS27 antibody detects endogenous levels of total MRPS27 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
428
Applicable Applications for anti-MRPS27 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:20000
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human MRPS27.
Preparation and Storage
Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells and HUVEC cells, using MRPS27 antibody.)

Western Blot (WB) (Western blot analysis of extracts from HeLa cells and HUVEC cells, using MRPS27 antibody.)
References
Nagase T., DNA Res. 3:321-329(1996). Koc E.C., Protein Sci. 10:471-481(2001).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49,150 Da
NCBI Official Full Name
28S ribosomal protein S27, mitochondrial isoform 1
NCBI Official Synonym Full Names
mitochondrial ribosomal protein S27
NCBI Official Symbol
MRPS27
NCBI Official Synonym Symbols
S27mt; MRP-S27
NCBI Protein Information
28S ribosomal protein S27, mitochondrial
UniProt Protein Name
28S ribosomal protein S27, mitochondrial
Protein Family
UniProt Gene Name
MRPS27
UniProt Synonym Gene Names
MRP-S27; S27mt

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2013]

Uniprot Description

RNA-binding component of the mitochondrial small ribosomal subunit (mt-SSU) that plays a role in mitochondrial protein synthesis (PubMed:22841715). Stimulates mitochondrial mRNA translation of subunit components of the mitochondrial electron transport chain (PubMed:22841715). Binds to the mitochondrial 12S rRNA (12S mt-rRNA) and tRNA(Glu) (PubMed:22841715). Involved also in positive regulation of cell proliferation and tumor cell growth (PubMed:28714366).

Research Articles on MRPS27

Similar Products

Product Notes

The MRPS27 mrps27 (Catalog #AAA854791) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The MRPS27 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MRPS27 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:20000. Researchers should empirically determine the suitability of the MRPS27 mrps27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MRPS27, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.