anti-Human, Mouse MBD3 Antibody | anti-MBD3 antibody

MBD3 Antibody

Cat. Num. AAA8510844

• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity-chromatography
• Synonyms: MBD3; MBD3 Antibody; methyl-CpG binding domain protein 3; Methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3; anti-MBD3 antibody

• Reactivity: Human, Mouse
• Specificity: MBD3 antibody detects endogenous levels of total MBD3
• Purity/Purification: Affinity-chromatography
• Form/Format: In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 291

• Applicable Applications for anti-MBD3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1:3000
• Immunogen: A synthesized peptide derived from human MBD3
• Preparation and Storage: Store at-20 degree C for 1 year

Western Blot (WB)

(Western blot analysis on Jurkat cell lysate using MBD3 Antibody)

Related Product Information for anti-MBD3 antibody

MBD3 methyl-CpG binding (MBD) proteins appear to bind differentially methylated domains (DMDs) of DNA, to recruit repression complexes, and to silence the locus. The methyl CpG binding domain of human MBD3 may not directly bind mCpG but does interact with the NuRD/Mi2 components HDAC1 and MTA2. Plays a role in histone deacetylation and nucleosome remodeling. Increased levels have been reported in lung cancer and glioblastomas and may be a therapeutic target.

NCBI and Uniprot Product Information

• NCBI GI #: 527498244
• NCBI GeneID: 53615
• NCBI Accession #: NP_001268382.1
• NCBI GenBank Nucleotide #: NM_001281453.1
• UniProt Accession #: O95983; Q6PIL9; Q6PJZ9; Q86XF4; A8K4B7; D6W5Z2
• Molecular Weight: 33kDa
• NCBI Official Full Name: methyl-CpG-binding domain protein 3 isoform 1
• NCBI Official Synonym Full Names: methyl-CpG binding domain protein 3
• NCBI Official Symbol: MBD3
• NCBI Protein Information: methyl-CpG-binding domain protein 3
• UniProt Protein Name: Methyl-CpG-binding domain protein 3
• Protein Family: Methyl-CpG-binding domain protein
• UniProt Gene Name: MBD3

NCBI Description

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. This gene belongs to a family of nuclear proteins which are characterized by the presence of a methyl-CpG binding domain (MBD). The encoded protein is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. Unlike the other family members, the encoded protein is not capable of binding to methylated DNA. The protein mediates the association of metastasis-associated protein 2 with the core histone deacetylase complex. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases.