Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Testing Data

anti-Human, Mouse IL-2Ralpha/CD25 Antibody | anti-IL2RA antibody

IL-2Ralpha/CD25 (Phospho-Ser268) Antibody

Gene Names
IL2RA; p55; CD25; IL2R; IMD41; TCGFR; IDDM10
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, ELISA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
IL-2Ralpha/CD25; IL-2Ralpha/CD25 (Phospho-Ser268) Antibody; CD25 antigen; IL-2 receptor alpha subunit; IL-2-RA; IL2-RA; Interleukin-2 receptor alpha chain precursor; TAC antigen; p55; anti-IL2RA antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse
Specificity
IL-2Ralpha/CD25 (Phospho-Ser268) antibody detects endogenous levels of IL-2Ralpha/CD25 only when phosphorylated at serine 268.
Purity/Purification
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
272
Applicable Applications for anti-IL2RA antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:10000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human IL-2Ralpha/CD25 around the phosphorylation site of serine 268 (R-K-SP-R-R).
All Sites
Human: Ser268
Mouse: Ser264
Preparation and Storage
Store at-20 degree C for 1 year.

Testing Data

Testing Data

Western Blot (WB)

(P-peptide-+ Western blot analysis of extracts from LOVO cells, using IL-2Ralpha/CD25 (Phospho-Ser268) antibody. Immunofluorescence analysis of HeLa cells, using IL-2Ralpha/CD25 (Phospho-Ser268) antibody.)

Western Blot (WB) (P-peptide-+ Western blot analysis of extracts from LOVO cells, using IL-2Ralpha/CD25 (Phospho-Ser268) antibody. Immunofluorescence analysis of HeLa cells, using IL-2Ralpha/CD25 (Phospho-Ser268) antibody.)
References
Nikaido T., Nature 311:631-635(1984). Bamborough P., Structure 2:839-851(1994). Stauber D.J., Proc. Natl. Acad. Sci. U.S.A. 103:2788-2793(2006).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,819 Da
NCBI Official Full Name
interleukin-2 receptor subunit alpha isoform 1
NCBI Official Synonym Full Names
interleukin 2 receptor subunit alpha
NCBI Official Symbol
IL2RA
NCBI Official Synonym Symbols
p55; CD25; IL2R; IMD41; TCGFR; IDDM10
NCBI Protein Information
interleukin-2 receptor subunit alpha
UniProt Protein Name
Interleukin-2 receptor subunit alpha
Protein Family
UniProt Gene Name
IL2RA
UniProt Synonym Gene Names
IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA

NCBI Description

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]

Uniprot Description

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

Research Articles on IL2RA

Similar Products

Product Notes

The IL2RA il2ra (Catalog #AAA858647) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The IL-2Ralpha/CD25 (Phospho-Ser268) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IL-2Ralpha/CD25 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000. Researchers should empirically determine the suitability of the IL2RA il2ra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-2Ralpha/CD25, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.