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Testing Data (P-peptide-+)

anti-Human, Mouse IGFBP-3 Antibody | anti-IGFBP-3 antibody

IGFBP-3 (Phospho-Ser183) Antibody

Gene Names
IGFBP3; IBP3; BP-53
Reactivity
Human, Mouse
Applications
Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
IGFBP-3; IGFBP-3 (Phospho-Ser183) Antibody; IBP-3; IBP3; IGF-binding protein 3; IGFBP3; Insulin-like growth factor binding protein 3 precursor; anti-IGFBP-3 antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse
Specificity
IGFBP-3 (Phospho-Ser183) antibody detects endogenous levels of IGFBP-3 only when phosphorylated at serine 183.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
291
Applicable Applications for anti-IGFBP-3 antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
IHC: 1:50-1:100
ELISA: 1:1000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human IGFBP-3 around the phosphorylation site of serine 183 (K-D-SP-Q-R).
All Sites
Human: Ser183
Mouse: Ser183
Preparation and Storage
Store at-20 degree C for 1 year.

Testing Data

(P-peptide-+)

Testing Data (P-peptide-+)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human brain tissue using IGFBP-3 (Phospho-Ser183) antibody. IGFBP-3 (Phospho-Ser183) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using IGFBP-3 (Phospho-Ser183) antibody. IGFBP-3 (Phospho-Ser183) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
References
C. Schmid, Am J Physiol Endocrinol Metab, Aug 1994; 267: E226-E233. Hanh Le, Am J Physiol Endocrinol Metab, May 2006; 290: E952-E960. C. A. Gibson, J Anim Sci, Jun 1999; 77: 1547-1557.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32,223 Da
NCBI Official Full Name
insulin-like growth factor-binding protein 3 isoform b
NCBI Official Synonym Full Names
insulin like growth factor binding protein 3
NCBI Official Symbol
IGFBP3
NCBI Official Synonym Symbols
IBP3; BP-53
NCBI Protein Information
insulin-like growth factor-binding protein 3
UniProt Protein Name
Insulin-like growth factor-binding protein 3
UniProt Gene Name
IGFBP3
UniProt Synonym Gene Names
IBP3; IBP-3; IGF-binding protein 3; IGFBP-3

NCBI Description

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

Research Articles on IGFBP-3

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Product Notes

The IGFBP-3 igfbp3 (Catalog #AAA859480) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The IGFBP-3 (Phospho-Ser183) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IGFBP-3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA). IHC: 1:50-1:100 ELISA: 1:1000. Researchers should empirically determine the suitability of the IGFBP-3 igfbp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IGFBP-3, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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