Immunofluorescence (IF)
(P-peptide-+ Immunofluorescence analysis of HuvEc cells, using GATA3 (Phospho-Ser308) antibody.)
anti-Human, Mouse GATA3 Antibody | anti-GATA3 antibody
GATA3 (Phospho-Ser308) Antibody
Gene Names
GATA3; HDR; HDRS
Reactivity
Human, Mouse
Applications
Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
GATA3; GATA3 (Phospho-Ser308) Antibody; GATA-binding factor 3; Trans-acting T-cell specific transcription factor GATA-3; anti-GATA3 antibody
Reactivity
Human, Mouse
Specificity
GATA3 (Phospho-Ser308) antibody detects endogenous levels of GATA3 only when phosphorylated at serine 308.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
444
Applicable Applications for anti-GATA3 antibody
Immunofluorescence (IF), ELISA (EIA)
Application Notes
IF: 1:100-1:500
ELISA: 1:10000
ELISA: 1:10000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human GATA3 around the phosphorylation site of serine 308 (R-L-SP-A-A).
All Sites
Human: Ser308
Mouse: Ser308
Mouse: Ser308
Preparation and Storage
Store at-20 degree C for 1 year.
Immunofluorescence (IF)
(P-peptide-+ Immunofluorescence analysis of HuvEc cells, using GATA3 (Phospho-Ser308) antibody.)
Immunofluorescence (IF)
(P-peptide-+ Immunofluorescence analysis of HuvEc cells, using GATA3 (Phospho-Ser308) antibody.)
References
Dorota Kurek, Development, Jan 2007; 134: 261-272. M. Andrew Nesbit, J. Biol. Chem., May 2004; 279: 22624-22634. Wei Xu, Blood, May 2007; 109: 4406-4414. David Grote, Development, Jan 2006; 133: 53-61.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,045 Da
NCBI Official Full Name
trans-acting T-cell-specific transcription factor GATA-3 isoform 1
NCBI Official Synonym Full Names
GATA binding protein 3
NCBI Official Symbol
GATA3
NCBI Official Synonym Symbols
HDR; HDRS
NCBI Protein Information
trans-acting T-cell-specific transcription factor GATA-3
UniProt Protein Name
Trans-acting T-cell-specific transcription factor GATA-3
Protein Family
UniProt Gene Name
GATA3
NCBI Description
This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]
Uniprot Description
Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.
Research Articles on GATA3
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Product Notes
The GATA3 gata3 (Catalog #AAA8509661) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The GATA3 (Phospho-Ser308) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GATA3 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA). IF: 1:100-1:500 ELISA: 1:10000. Researchers should empirically determine the suitability of the GATA3 gata3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GATA3, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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