anti-Human, Mouse ERCC1 Antibody | anti-ERCC1 antibody

ERCC1 Antibody

Cat. Num. AAA851887

• Gene Names: ERCC1; UV20; COFS4; RAD10
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: ERCC1; ERCC1 Antibody; UV20; DNA REPAIR DEFECT UV-20 OF CHINESE HAMSTER OVARY CELLS; COMPLEMENTATION OF EXCISION-REPAIR; COMPLEMENTING DEFECTIVE IN CHINESE HAMSTER; excision repair cross-complementing 1 isoform 1; excision repair cross-complementing 1 isofrom 2; excision repair protein; anti-ERCC1 antibody

• Reactivity: Human, Mouse
• Specificity: ERCC1 antibody detects endogenous levels of total ERCC1 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 273

• Applicable Applications for anti-ERCC1 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:5000
• Immunogen: The antiserum was produced against synthesized peptide derived from human ERCC1.
• Preparation and Storage: Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(Peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ERCC1 antibody.)

Western Blot (WB)

(Western blot analysis of extracts from NIH/3T3 cells, using ERCC1 antibody.)

References

Kan-Tai Hsia, Development, Jan 2003; 130: 369. Jim Selfridge, Nucleic Acids Res., Nov 2001; 29: 4541-4550. Cha-Kyung Youn, Cancer Res., Jul 2004; 64: 4849-4857. Teresa A. Motycka, J. Biol. Chem., Apr 2004; 279: 13634-13639.

NCBI and Uniprot Product Information

• NCBI GI #: 260593725
• NCBI GeneID: 2067
• NCBI Accession #: NP_001159521.1
• NCBI GenBank Nucleotide #: NM_001166049.1
• UniProt Accession #: P07992; Q7Z7F5; Q96S40; B2RC01; B3KRR0
• Molecular Weight: 25,211 Da
• NCBI Official Full Name: DNA excision repair protein ERCC-1 isoform 3
• NCBI Official Synonym Full Names: ERCC excision repair 1, endonuclease non-catalytic subunit
• NCBI Official Symbol: ERCC1
• NCBI Official Synonym Symbols: UV20; COFS4; RAD10
• NCBI Protein Information: DNA excision repair protein ERCC-1
• UniProt Protein Name: DNA excision repair protein ERCC-1
• Protein Family: DNA excision repair protein
• UniProt Gene Name: ERCC1

NCBI Description

The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009]

Uniprot Description

Isoform 1: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.