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Western Blot (WB) (Western blot analysis of extracts from 293 cells, treated with PMA (125ng/ml, 30mins), using CD5 (Ab-453) antibody.)

anti-Human, Mouse CD5 Antibody | anti-CD5 antibody

CD5 (Ab-Tyr453) Antibody

Gene Names
CD5; T1; LEU1
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
CD5; CD5 (Ab-Tyr453) Antibody; LEU1; Lymphocyte antigen CD5; Lymphocyte glycoprotein Ly-1; Lymphocyte glycoprotein T1/Leu-1; Lyt-1; T-cell surface glycoprotein CD5 precursor; anti-CD5 antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse
Specificity
CD5 (Ab-453) antibody detects endogenous levels of total CD5 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
438
Applicable Applications for anti-CD5 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:20000
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human CD5 around the phosphorylation site of tyrosine 453 (N-E-YP-S-Q).
Preparation and Storage
Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from 293 cells, treated with PMA (125ng/ml, 30mins), using CD5 (Ab-453) antibody.)

Western Blot (WB) (Western blot analysis of extracts from 293 cells, treated with PMA (125ng/ml, 30mins), using CD5 (Ab-453) antibody.)
References
Masanori Taniguchi, Blood, Feb 1998; 91: 1145-1151. David Kaplan, PNAS, Nov 2001; 98: 13850. SB Ebeling, J. Immunol., Dec 1993; 151: 6891. Glenn H. Cantor, J. Virol., Feb 2001; 75: 1689-1696.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
921
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
54,578 Da
NCBI Official Full Name
T-cell surface glycoprotein CD5 isoform 2
NCBI Official Synonym Full Names
CD5 molecule
NCBI Official Symbol
CD5
NCBI Official Synonym Symbols
T1; LEU1
NCBI Protein Information
T-cell surface glycoprotein CD5
UniProt Protein Name
T-cell surface glycoprotein CD5
Protein Family
UniProt Gene Name
CD5
UniProt Synonym Gene Names
LEU1

NCBI Description

This gene encodes a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2016]

Uniprot Description

May act as a receptor in regulating T-cell proliferation.

Research Articles on CD5

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Product Notes

The CD5 cd5 (Catalog #AAA858930) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The CD5 (Ab-Tyr453) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:20000. Researchers should empirically determine the suitability of the CD5 cd5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD5, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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