Testing Data
(P-peptide-+)
anti-Human, Mouse 4E-BP1 Antibody | anti-4E-BP1 antibody
4E-BP1 (Phospho-Thr69) Antibody
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:40000
Mouse: Ser1189
Testing Data
(P-peptide-+)
Testing Data
(P-peptide-+)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using SEPARASE (Phospho-Ser801) antibody. Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using SEPARASE (Phospho-Ser801) antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using SEPARASE (Phospho-Ser801) antibody. Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using SEPARASE (Phospho-Ser801) antibody.)
Immunofluorescence (IF)
(P-peptide-+ Immunofluorescence analysis of HuvEc cells, using SEPARASE (Phospho-Ser801) antibody.)
Immunofluorescence (IF)
(P-peptide-+ Immunofluorescence analysis of HuvEc cells, using SEPARASE (Phospho-Ser801) antibody.)
NCBI and Uniprot Product Information
NCBI Description
Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM, Nov 2010]
Uniprot Description
Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.
Research Articles on 4E-BP1
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Product Notes
The 4E-BP1 espl1 (Catalog #AAA853512) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The 4E-BP1 (Phospho-Thr69) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's 4E-BP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000. Researchers should empirically determine the suitability of the 4E-BP1 espl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "4E-BP1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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