Perforin ELISpot Set | PRF1 elispot set

Human Perforin ELISpot Set

Cat. Num. AAA335577

• Gene Names: PRF1; P1; PFP; FLH2; PFN1; HPLH2
• Reactivity: Human
• Synonyms: Perforin; Human Perforin ELISpot Set; Perforin (without plate); PRF1 elispot set

• Reactivity: Human
• Specificity: Recognizes natural human Perforin
• Sequence Length: 555

• Samples: Dilute PBMC in culture medium and stimulator of interest (i.e. Sample, Vaccine, Peptide pool or infected cells) to give an appropriate cell number per 100ml. Optimal assay performances are observed between 1x105 and 2.5x105 cells per 100ml. Stimulators and incubation times can be varied depending on the frequency of cytokine producing cells and therefore should be optimised by the testing laboratory.

Related Product Information for PRF1 elispot set

MyBioSource ELISpot Sets include capture and detection antibodies, Streptavidin - Alkaline Phosphatase conjugated, BSA, BCIP/NTB, blocking reagent.

Product Categories/Family for PRF1 elispot set

Human ELISpot Set

References

Van Gulck, E. R. et al, J. Virol,2008; 82(7): 3561-3573.

NCBI and Uniprot Product Information

• NCBI GI #: 189847
• NCBI GeneID: 5551
• UniProt Accession #: P14222; Q59F57; Q86WX7; B2R6X4
• Molecular Weight: 61,377 Da
• NCBI Official Full Name: perforin
• NCBI Official Synonym Full Names: perforin 1 (pore forming protein)
• NCBI Official Symbol: PRF1
• NCBI Official Synonym Symbols: P1; PFP; FLH2; PFN1; HPLH2
• NCBI Protein Information: perforin-1
• UniProt Protein Name: Perforin-1
• Protein Family: Perforin
• UniProt Gene Name: PRF1
• UniProt Synonym Gene Names: PFP; P1; PFP
• UniProt Entry Name: PERF_HUMAN

NCBI Description

The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

PRF1: Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. Monomer, as sobluble protein. Homooligomer. Oligomerization is required for pore formation. Repressed by contact with target cells. Belongs to the complement C6/C7/C8/C9 family.

Protein type: Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 10q22

Cellular Component: membrane; cytoplasmic membrane-bound vesicle; extracellular region; plasma membrane; integral to membrane

Molecular Function: protein binding; calcium ion binding; wide pore channel activity

Biological Process: formation of immunological synapse; apoptosis; cytolysis; defense response to tumor cell; cellular defense response; immune response to tumor cell; transmembrane transport; defense response to virus; protein homooligomerization

Disease: Lymphoma, Non-hodgkin, Familial; Aplastic Anemia; Hemophagocytic Lymphohistiocytosis, Familial, 2