IL-4 elispot kit

Human IL-4 ELISpot Kit

Cat. Num. AAA335695

• Reactivity: Human. No cross reactivity with other human cytokines.
• Synonyms: IL-4; Human IL-4 ELISpot Kit; IL-4 elispot kit

• Reactivity: Human. No cross reactivity with other human cytokines.
• Specificity: The assay recognizes natural human IL-4. To define specificity, several proteins were tested for cross reactivity. There was no cross reactivity observed for any protein tested (IL-1alpha, IL-1beta, IL-10 IL-12, IFNgamma, IL-2, IL-6, TNFalpha, IL-8 and IL-13) This testing was performed using the equivalent human IL-4 antibody pair in an ELISA assay.
• Sequence Length: 133

• Intended Uses: ELISpot is a highly specific immunoassay for the analysis of cytokine and other soluble molecule production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation and the comparison of such frequency against a specific treatment or pathological state. The ELISpot assay constitutes an ideal tool in the investigation of Th1 / Th2 responses, vaccine development, viral infection monitoring and treatment, cancerology, infectious disease, autoimmune diseases and transplantation. Utilising sandwich immuno-enzyme technology, ELISpot assays can detect both secreted cytokines and single cells that simultaneously produce multiple cytokines. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

Related Product Information for IL-4 elispot kit

Principle of the assay: A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtitre plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and washed. Cell suspension and stimulant are added and the plate incubated allowing the specific antibodies to bind any analytes produced. Cells are then removed by washing prior to the addition of Biotinylated detection antibodies which bind to the previously captured analyte. Enzyme conjugated streptavidin is then added binding to the detection antibodies. Following incubation and washing substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using a microscope.

Product Categories/Family for IL-4 elispot kit

Human ELISpot Kit

References

Hudak S. et al., J. Immuno., 2002; 169(3): 1189 - 1196
Sauce D. et al., Blood, 2002; 99(4) : 1165 - 1173

NCBI and Uniprot Product Information

• NCBI GI #: 55742622
• NCBI GeneID: 397225
• NCBI Accession #: NP_999288.1
• NCBI GenBank Nucleotide #: NM_214123.1
• Molecular Weight: 15,023 Da
• NCBI Official Full Name: interleukin-4
• NCBI Official Symbol: IL4
• NCBI Protein Information: interleukin-4
• UniProt Protein Name: Interleukin-4
• UniProt Gene Name: IL4
• UniProt Synonym Gene Names: IL-4; BSF-1
• UniProt Entry Name: IL4_PIG

Uniprot Description

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages.