Human Wingless Type MMTV Integration Site Family, Member 10A (WNT10A) ELISA Kit | WNT10A elisa kit

Human Wingless Type MMTV Integration Site Family, Member 10A (WNT10A) ELISA Kit

Cat. Num. AAA2707163

• Gene Names: WNT10A; OODD; SSPS; STHAG4
• Reactivity: Human
• Synonyms: Wingless Type MMTV Integration Site Family; Member 10A (WNT10A); Human Wingless Type MMTV Integration Site Family; Member 10A (WNT10A) ELISA Kit; WNT10A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 10A (WNT10A).; No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 10A (WNT10A) and analogues was observed.
• Sequence Length: 417

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.061ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 10A (WNT10A) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 10A (WNT10A) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WNT10A elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 10A (WNT10A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 10A (WNT10A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 10A (WNT10A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wingless Type MMTV Integration Site Family, Member 10A (WNT10A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119591065
• NCBI GeneID: 80326
• UniProt Accession #: Q9GZT5; Q53S44; Q96TA7; Q9H7S8
• Molecular Weight: 46,444 Da
• NCBI Official Full Name: wingless-type MMTV integration site family, member 10A
• NCBI Official Synonym Full Names: Wnt family member 10A
• NCBI Official Symbol: WNT10A
• NCBI Official Synonym Symbols: OODD; SSPS; STHAG4
• NCBI Protein Information: protein Wnt-10a
• UniProt Protein Name: Protein Wnt-10a
• UniProt Gene Name: WNT10A

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is strongly expressed in the cell lines of promyelocytic leukemia and Burkitt's lymphoma. In addition, it and another family member, the WNT6 gene, are strongly coexpressed in colorectal cancer cell lines. The gene overexpression may play key roles in carcinogenesis through activation of the WNT-beta-catenin-TCF signaling pathway. This gene and the WNT6 gene are clustered in the chromosome 2q35 region. [provided by RefSeq, Jul 2008]

Uniprot Description

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.