Human WD Repeat And SOCS Box Containing Protein 1 (WSB1) ELISA Kit | WSB1 elisa kit

Human WD Repeat And SOCS Box Containing Protein 1 (WSB1) ELISA Kit

Cat. Num. AAA2707773

• Gene Names: WSB1; SWIP1; WSB-1
• Reactivity: Human
• Synonyms: WD Repeat And SOCS Box Containing Protein 1 (WSB1); Human WD Repeat And SOCS Box Containing Protein 1 (WSB1) ELISA Kit; SWIP1; WSB-1; SOCS box-containing WD protein SWiP-1; WSB1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of WD Repeat And SOCS Box Containing Protein 1 (WSB1).; No significant cross-reactivity or interference between WD Repeat And SOCS Box Containing Protein 1 (WSB1) and analogues was observed.
• Sequence Length: 421

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.121ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WD Repeat And SOCS Box Containing Protein 1 (WSB1) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WD Repeat And SOCS Box Containing Protein 1 (WSB1) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WSB1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WD Repeat And SOCS Box Containing Protein 1 (WSB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WD Repeat And SOCS Box Containing Protein 1 (WSB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WD Repeat And SOCS Box Containing Protein 1 (WSB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of WD Repeat And SOCS Box Containing Protein 1 (WSB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 18677720
• NCBI GeneID: 26118
• NCBI GenBank Nucleotide #: NP_056441.6
• UniProt Accession #: Q9Y6I7
• NCBI Official Full Name: WD repeat and SOCS box-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: WD repeat and SOCS box containing 1
• NCBI Official Symbol: WSB1
• NCBI Official Synonym Symbols: SWIP1; WSB-1
• NCBI Protein Information: WD repeat and SOCS box-containing protein 1
• UniProt Protein Name: WD repeat and SOCS box-containing protein 1
• Protein Family: WD repeat and SOCS box-containing protein
• UniProt Gene Name: WSB1
• UniProt Synonym Gene Names: SWIP1; WSB-1
• UniProt Entry Name: WSB1_HUMAN

NCBI Description

This gene encodes a member of the WD-protein subfamily. This protein shares a high sequence identity to mouse and chick proteins. It contains several WD-repeats spanning most of the protein and an SOCS box in the C-terminus. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

WSB1: Probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes type II iodothyronine deiodinase/DIO2. Confers constitutive instability to HIPK2 through proteasomal degradation. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 17q11.1

Cellular Component: intracellular

Molecular Function: protein binding

Biological Process: protein ubiquitination