Human Vitamin D Binding Protein ELISA Kit | GC elisa kit

Human Vitamin D Binding Protein (GC Globulin) ELISA Kit

Cat. Num. AAA564062

• Gene Names: GC; DBP; GRD3; VDBG; VDBP; DBP/GC
• Reactivity: Human
• Synonyms: Vitamin D Binding Protein; Human Vitamin D Binding Protein (GC Globulin) ELISA Kit; GC elisa kit

• Reactivity: Human

• Samples: Human samples
• Assay Type: Sandwich
• Preparation and Storage: Store at 4 degree C.

Related Product Information for GC elisa kit

Intended Uses: The VDBP test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring VDBP in human biological samples. If the ELISA is to be used outside the intended use, the user may need to optimize for said use.

Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Vitamin D binding protein (VDBP) present in samples reacts with the anti-VDBP antibodies, which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-VDBP antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound VDBP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of VDBP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of VDBP in the test sample. The quantity of VDBP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Product Categories/Family for GC elisa kit

ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 32483410
• NCBI GeneID: 2638
• NCBI Accession #: NP_000574.2
• NCBI GenBank Nucleotide #: NM_000583.3
• UniProt Accession #: P02774; Q16309; Q16310; Q53F31; Q6GTG1; B4DPP2; Q6LDC6
• Molecular Weight: 52,964 Da
• NCBI Official Full Name: vitamin D-binding protein isoform 1
• NCBI Official Synonym Full Names: group-specific component (vitamin D binding protein)
• NCBI Official Symbol: GC
• NCBI Official Synonym Symbols: DBP; GRD3; VDBG; VDBP; DBP/GC
• NCBI Protein Information: vitamin D-binding protein; VDB; gc-globulin; OTTHUMP00000160364; OTTHUMP00000218973; OTTHUMP00000218974; OTTHUMP00000218975; vitamin D-binding alpha-globulin
• UniProt Protein Name: Vitamin D-binding protein
• Protein Family: Vitamin D-binding protein
• UniProt Gene Name: GC
• UniProt Entry Name: VTDB_HUMAN

NCBI Description

The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq]

Uniprot Description

GC: Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q12-q13

Cellular Component: extracellular space; lysosomal lumen; extracellular region; cytosol

Molecular Function: vitamin D binding; actin binding; vitamin transporter activity

Biological Process: steroid metabolic process; vitamin transport; vitamin D metabolic process

Disease: Graves Disease, Susceptibility To, 1