Human VEGFR2/KDR ELISA Kit | KDR elisa kit

Human VEGFR2/KDR PicoKine ELISA Kit

Cat. Num. AAA175978

• Gene Names: KDR; FLK1; CD309; VEGFR; VEGFR2
• Reactivity: Human
• Synonyms: VEGFR2/KDR; Human VEGFR2/KDR PicoKine ELISA Kit; Vascular endothelial growth factor receptor 2; kinase insert domain receptor (a type III receptor tyrosine kinase); CD309; Fetal liver kinase 1; FLK1; KDR; Kinase insert domain receptor; Kinase insert domain receptor (a type III receptor tyrosine kinase); Protein tyrosine kinase receptor Flk 1; Tyrosine kinase growth factor receptor; VEGFR; VEGFR 2; VEGFR2; KDR elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human VEGFR2
• Sequence Length: 1356

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: < 4pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human VEGFR2/KDR PicoKine ELISA Kit standard curve )

Related Product Information for KDR elisa kit

Principle of the assay: human VEGFR2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for VEGFR2 has been precoated onto 96-well plates. Standards(NSO, A20-E764) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGFR2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human VEGFR2 amount of sample captured in plate.
Background: Vascular endothelial growth factor receptor 2 (VEGFR-2) also known as Kinase insert domain receptor (KDR, a type III receptor tyrosine kinase) is a VEGF receptor. Through in situ hybridization of a genomic DNA probe to metaphase chromosomes, VEGFR2 was localized to 4q11-->q12. VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells.

NCBI and Uniprot Product Information

• NCBI GI #: 9087218
• NCBI GeneID: 3791
• UniProt Accession #: P35968; O60723; Q14178; A2RRS0; B5A925; C5IFA0
• Molecular Weight: 79,634 Da
• NCBI Official Full Name: Vascular endothelial growth factor receptor 2
• NCBI Official Synonym Full Names: kinase insert domain receptor (a type III receptor tyrosine kinase)
• NCBI Official Symbol: KDR
• NCBI Official Synonym Symbols: FLK1; CD309; VEGFR; VEGFR2
• NCBI Protein Information: vascular endothelial growth factor receptor 2; soluble VEGFR2; fetal liver kinase 1; fetal liver kinase-1; protein-tyrosine kinase receptor Flk-1; tyrosine kinase growth factor receptor
• UniProt Protein Name: Vascular endothelial growth factor receptor 2
• Protein Family: Vascular endothelial growth factor receptor
• UniProt Gene Name: KDR
• UniProt Synonym Gene Names: FLK1; VEGFR2; VEGFR-2; FLK-1; KDR
• UniProt Entry Name: VGFR2_HUMAN

NCBI Description

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009]

Uniprot Description

VEGFR2: a receptor tyrosine kinase of the VEGFR family. High affinity receptor for VEGF and VEGF-C. Ligand binding induces autophosphorylation and activation. Activated receptor recruits proteins including Shc, GRB2, PI3K, Nck, SHP-1 and SHP-2. Plays a key role in vascular development and regulation of vascular permeability.

Protein type: Protein kinase, TK; Membrane protein, integral; EC 2.7.10.1; Kinase, protein; Protein kinase, tyrosine (receptor); TK group; VEGFR family

Chromosomal Location of Human Ortholog: 4q11-q12

Cellular Component: Golgi apparatus; integral to plasma membrane; endoplasmic reticulum; cytoplasmic membrane-bound vesicle; early endosome; extracellular region; plasma membrane; cell junction; nucleus; endosome; external side of plasma membrane; lipid raft

Molecular Function: integrin binding; vascular endothelial growth factor receptor activity; protein binding; protein-tyrosine kinase activity; growth factor binding; Hsp90 protein binding; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of positive chemotaxis; peptidyl-tyrosine phosphorylation; viral reproduction; protein amino acid autophosphorylation; cell maturation; calcium ion homeostasis; regulation of cell shape; positive regulation of MAPKKK cascade; positive regulation of focal adhesion formation; ovarian follicle development; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; angiogenesis; vasculogenesis; endothelial cell differentiation; cell fate commitment; embryonic hemopoiesis; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of angiogenesis; branching morphogenesis of a tube; cell migration during sprouting angiogenesis; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; lymph vessel development; surfactant homeostasis; alveolus development; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration; positive regulation of nitric-oxide synthase biosynthetic process; negative regulation of apoptosis

Disease: Hemangioma, Capillary Infantile