Human V type proton ATPase 116 kDa subunit a isoform 1 (ATP6V0A1) ELISA Kit | ATP6V0A1 elisa kit

Human V type proton ATPase 116 kDa subunit a isoform 1 (ATP6V0A1) ELISA Kit

Cat. Num. AAA7245132

• Gene Names: ATP6V0A1; a1; Stv1; VPP1; Vph1; ATP6N1; ATP6N1A
• Reactivity: Human
• Synonyms: V type proton ATPase 116 kDa subunit a isoform 1 (ATP6V0A1); Human V type proton ATPase 116 kDa subunit a isoform 1 (ATP6V0A1) ELISA Kit; ATP6V0A1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATP6V0A1. No significant cross-reactivity or interference between ATP6V0A1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATP6V0A1 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATP6V0A1 elisa kit

Intended Uses: This ATP6V0A1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATP6V0A1. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: ATP6V0A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ATP6V0A1 antibody and an ATP6V0A1-HRP conjugate. The assay sample and buffer are incubated together with ATP6V0A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATP6V0A1 concentration since ATP6V0A1 from samples and ATP6V0A1-HRP conjugate compete for the anti-ATP6V0A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP6V0A1 from the sample, fewer sites are left to bind ATP6V0A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATP6V0A1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ATP6V0A1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 59803038
• NCBI GeneID: 535
• Molecular Weight: 96,600 Da
• NCBI Official Full Name: V-type proton ATPase 116 kDa subunit a isoform 1
• NCBI Official Synonym Full Names: ATPase, H+ transporting, lysosomal V0 subunit a1
• NCBI Official Symbol: ATP6V0A1
• NCBI Official Synonym Symbols: a1; Stv1; VPP1; Vph1; ATP6N1; ATP6N1A
• NCBI Protein Information: V-type proton ATPase 116 kDa subunit a isoform 1; V-ATPase 116 kDa; vacuolar proton pump subunit 1; vacuolar proton pump, subunit 1; vacuolar-type H(+)-ATPase 115 kDa subunit; vacuolar adenosine triphosphatase subunit Ac116; vacuolar proton translocating ATPase 116 kDa subunit A; H(+)-transporting two-sector ATPase, 116 kDa accessory protein A1; clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit; ATPase, H+ transporting, lysosomal non-catalytic accessory protein 1 (110/116kD); ATPase, H+ transporting, lysosomal (vacuolar proton pump) non-catalytic accessory protein 1A (110/116kD)
• UniProt Protein Name: V-type proton ATPase 116 kDa subunit a isoform 1
• Protein Family: V-type proton ATPase
• UniProt Gene Name: ATP6V0A1
• UniProt Synonym Gene Names: ATP6N1; ATP6N1A; VPP1; V-ATPase 116 kDa isoform a1
• UniProt Entry Name: VPP1_HUMAN

NCBI Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three A subunit proteins and the encoded protein is associated with clathrin-coated vesicles. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP6V0A1: Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle. Belongs to the V-ATPase 116 kDa subunit family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter; Motility/polarity/chemotaxis; Membrane protein, multi-pass; Membrane protein, integral; Transporter, iron; Transporter, ion channel; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; Golgi apparatus; phagocytic vesicle membrane; intracellular membrane-bound organelle; cytoplasm; melanosome; integral to membrane; plasma membrane; endosome membrane; vacuolar proton-transporting V-type ATPase complex

Molecular Function: protein binding; hydrogen ion transporting ATPase activity, rotational mechanism; ATPase binding

Biological Process: interaction with host; vacuolar acidification; ATP synthesis coupled proton transport; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; transmembrane transport