Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human USP6 N-terminal-like protein (USP6NL) Standard Curve.)
Human USP6 N-Terminal-Like Protein (USP6NL) ELISA Kit | USP6NL elisa kit
Human USP6 N-Terminal-Like Protein (USP6NL) ELISA Kit
Gene Names
USP6NL; RNTRE; TRE2NL; USP6NL-IT1
Reactivity
Human
Synonyms
USP6 N-Terminal-Like Protein (USP6NL); Human USP6 N-Terminal-Like Protein (USP6NL) ELISA Kit; USP6NL; KIAA0019; RNTRE; TRE2NL; related to the N terminus of tre; USP6NL elisa kit
Reactivity
Human
Specificity
Human USP6 N-terminal-like protein (USP6NL) ELISA Kit has high sensitivity and excellent specificity for detection of Human USP6NL. No significant cross-reactivity or interference between Human USP6NL and analogues was observed.
Sequence Length
845
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human USP6 N-terminal-like protein (USP6NL) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human USP6 N-terminal-like protein (USP6NL) Standard Curve.)
Related Product Information for USP6NL elisa kit
Description: This Human USP6 N-terminal-like protein (USP6NL) ELISA Kit employs a two-site sandwich ELISA to quantitate USP6NL in samples. An antibody specific for USP6NL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any USP6NL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for USP6NL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of USP6NL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: HAUSPis a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates.Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, HAUSP acitivity generally stabilizes its substrate proteins. HAUSP is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53. Normally, p53 levels are kept low in part due to Mdm2-mediated ubiquitylation and degradation of p53. Interestingly, in response to oncogenic insults, HAUSP can deubiquitinate p53 and protect p53 from Mdm2-mediated degradation, indicating that it may possess a tumor suppressor function for the immediate stabilization of p53 in response to stress.
Background: HAUSPis a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates.Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, HAUSP acitivity generally stabilizes its substrate proteins. HAUSP is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53. Normally, p53 levels are kept low in part due to Mdm2-mediated ubiquitylation and degradation of p53. Interestingly, in response to oncogenic insults, HAUSP can deubiquitinate p53 and protect p53 from Mdm2-mediated degradation, indicating that it may possess a tumor suppressor function for the immediate stabilization of p53 in response to stress.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
USP6 N-terminal-like protein isoform 2
NCBI Official Synonym Full Names
USP6 N-terminal like
NCBI Official Symbol
USP6NL
NCBI Official Synonym Symbols
RNTRE; TRE2NL; USP6NL-IT1
NCBI Protein Information
USP6 N-terminal-like protein
UniProt Protein Name
USP6 N-terminal-like protein
Protein Family
UniProt Gene Name
USP6NL
UniProt Synonym Gene Names
KIAA0019; RN-tre
UniProt Entry Name
US6NL_HUMAN
Uniprot Description
USP6NL: a widely-expressed GTPase activating protein (GAP) for RAB5A. Involved in endosomal trafficking, and localizes to the centrosome. In complex with Eps8 inhibits internalization of EGFR.
Protein type: GAPs, Rab; GAPs; Ubiquitin-specific protease
Chromosomal Location of Human Ortholog: 10p13
Cellular Component: Golgi apparatus; cytoplasmic membrane-bound vesicle; plasma membrane; cytoplasmic vesicle
Molecular Function: Rab GTPase binding
Biological Process: plasma membrane to endosome transport; virus assembly; Golgi organization and biogenesis
Research Articles on USP6NL
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Product Notes
The Human USP6NL usp6nl (Catalog #AAA9713748) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713748 ELISA Kit recognizes Human USP6NL. It is sometimes possible for the material contained within the vial of "USP6 N-Terminal-Like Protein (USP6NL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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