Human Uromodulin (UMOD) ELISA Kit | UMOD elisa kit

Human Uromodulin (UMOD) ELISA Kit

Cat. Num. AAA3801105

• Gene Names: UMOD; THP; FJHN; HNFJ; THGP; HNFJ1; MCKD2; ADMCKD2
• Reactivity: Human
• Synonyms: Uromodulin (UMOD); Human Uromodulin (UMOD) ELISA Kit; UMOD elisa kit

• Reactivity: Human
• Sequence Length: 640

• Samples: Serum, plasma, cell culture supernate and other biological fluids
• Assay Type: Sandwich
• Detection Range: 2.5 mug/ml-40 mug/ml
• Sensitivity: 0.1 mug/ml

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for UMOD elisa kit

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of UMODin the sample, This UMOD Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus UMODconcentration. The concentration of UMODin the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 59850812
• NCBI GeneID: 7369
• NCBI Accession #: NP_003352.2
• NCBI GenBank Nucleotide #: NP_003352.2
• UniProt Accession #: P07911
• NCBI Official Full Name: uromodulin isoform a preproprotein
• NCBI Official Synonym Full Names: uromodulin
• NCBI Official Symbol: UMOD
• NCBI Official Synonym Symbols: THP; FJHN; HNFJ; THGP; HNFJ1; MCKD2; ADMCKD2
• NCBI Protein Information: uromodulin
• UniProt Protein Name: Uromodulin
• Protein Family: Uromodulin
• UniProt Gene Name: UMOD
• UniProt Synonym Gene Names: THP
• UniProt Entry Name: UROM_HUMAN

NCBI Description

The protein encoded by this gene is the most abundant protein in mammalian urine under physiological conditions. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. This protein may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of this protein in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the renal disorders medullary cystic kidney disease-2 (MCKD2), glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI), and familial juvenile hyperuricemic nephropathy (FJHN). Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

UMOD: Uromodulin: Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure providing the water barrier permeability. May serve as a receptor for binding and endocytosis for cytokines (IL-1, IL-2) and TNF. Facilitates neutrophil migration across renal epithelial. Defects in UMOD are the cause of familial juvenile hyperuricemic nephropathy type 1 (HNFJ1). HNFJ1 is a renal disease characterized by juvenil onset of hyperuricemia, polyuria, progressive renal failure, and gout. The disease is associated with interstitial pathological changes resulting in fibrosis. Defects in UMOD are the cause of medullary cystic kidney disease type 2 (MCKD2). MCKD2 is a form of tubulointerstitial nephropathy characterized by formation of renal cysts at the corticomedullary junction. It is characterized by adult onset of impaired renal function and salt wasting resulting in end-stage renal failure by the sixth decade. Defects in UMOD are the cause of glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI). GCKDHI is a renal disorder characterized by a cystic dilation of Bowman space, a collapse of glomerular tuft, and hyperuricemia due to low fractional excretion of uric acid and severe impairment of urine concentrating ability. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 16p12.3

Cellular Component: Golgi apparatus; spindle pole; extracellular space; extrinsic to membrane; basolateral plasma membrane; apical plasma membrane; cytoplasmic vesicle; lipid raft

Molecular Function: IgG binding; calcium ion binding

Biological Process: heterophilic cell adhesion; response to organic substance; negative regulation of cell proliferation; leukocyte adhesion; cellular defense response; excretion; chemical homeostasis

Disease: Hyperuricemic Nephropathy, Familial Juvenile, 1; Glomerulocystic Kidney Disease With Hyperuricemia And Isosthenuria; Medullary Cystic Kidney Disease 2