Human Ubiquitin Specific Peptidase 8 (USP8) ELISA Kit | USP8 elisa kit

Human Ubiquitin Specific Peptidase 8 (USP8) ELISA Kit

Cat. Num. AAA450099

• Gene Names: USP8; UBPY; SPG59; HumORF8
• Reactivity: Human
• Synonyms: Ubiquitin Specific Peptidase 8 (USP8); Human Ubiquitin Specific Peptidase 8 (USP8) ELISA Kit; hnRNP K; HNRNPK; Transformation up-regulated nuclear protein; TUNP; USP8 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of USP8. No significant cross-reactivity or interference between USP8 and analogues was observed.
• Sequence Length: 1118

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.101ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level USP8 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level USP8 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for USP8 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of USP8 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to USP8. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to USP8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain USP8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of USP8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 190684692
• NCBI GeneID: 9101
• NCBI Accession #: NP_001122082.1
• NCBI GenBank Nucleotide #: NM_001128610.2
• UniProt Accession #: P40818; Q2TB31; Q7Z3U2; Q86VA0; Q8IWI7; B4DKA8
• Molecular Weight: 115,080 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 8 isoform a
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 8
• NCBI Official Symbol: USP8
• NCBI Official Synonym Symbols: UBPY; SPG59; HumORF8
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 8
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 8
• UniProt Gene Name: USP8
• UniProt Synonym Gene Names: KIAA0055; UBPY; hUBPy

NCBI Description

This gene encodes a protein that belongs to the ubiquitin-specific processing protease family of proteins. The encoded protein is thought to regulate the morphology of the endosome by ubiquitination of proteins on this organelle and is involved in cargo sorting and membrane trafficking at the early endosome stage. This protein is required for the cell to enter the S phase of the cell cycle and also functions as a positive regulator in the Hedgehog signaling pathway in development. Pseudogenes of this gene are present on chromosomes 2 and 6. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1.