Typical Testing Data/Standard Curve (for reference only)
Human Ubiquitin Like Modifier Activating Enzyme 2 (UBA2) ELISA Kit | UBA2 elisa kit
Human Ubiquitin Like Modifier Activating Enzyme 2 (UBA2)(Sandwich ELISA) ELISA Kit
Gene Names
UBA2; ARX; SAE2; HRIHFB2115
Reactivity
Human
Applications
ELISA
Synonyms
Ubiquitin Like Modifier Activating Enzyme 2 (UBA2); Human Ubiquitin Like Modifier Activating Enzyme 2 (UBA2)(Sandwich ELISA) ELISA Kit; ELISA Kit for Ubiquitin Like Modifier Activating Enzyme 2 (UBA2); UBA2/Ubiquitin Like Modifier Activating Enzyme 2; SAE2; ARX; SUMO1 Activating Enzyme Subunit 2; Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B; SUMO-activating enzyme subunit 2; UBA2 elisa kit
Reactivity
Human
Specificity
Reactive with Human Ubiquitin Like Modifier Activating Enzyme 2 (UBA2)
This assay has high sensitivity and excellent specificity for detection of Ubiquitin Like Modifier Activating Enzyme 2 (UBA2). No significant cross-reactivity or interference between Ubiquitin Like Modifier Activating Enzyme 2 (UBA2) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Ubiquitin Like Modifier Activating Enzyme 2 (UBA2). No significant cross-reactivity or interference between Ubiquitin Like Modifier Activating Enzyme 2 (UBA2) and analogues was observed.
Applicable Applications for UBA2 elisa kit
ELISA (EIA)
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Assay Type
Sandwich ELISA
Sample Types
tissue homogenates, cell lysates and other biological fluids
Detection Method
Double-antibody Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
0.059ng/mL
Assay Length
3 h
Sample Size
100ul (Please refer to the manual for the specific data.)
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Like Modifier Activating Enzyme 2 (UBA2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Like Modifier Activating Enzyme 2 (UBA2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Like Modifier Activating Enzyme 2 (UBA2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Assay Procedure Summary
1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 50ul Stop Solution. Read at 450nm immediately.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 50ul Stop Solution. Read at 450nm immediately.
Test Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thyroxine Binding Globulin (TBG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thyroxine Binding Globulin (TBG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thyroxine Binding Globulin (TBG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Thyroxine Binding Globulin (TBG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Preparation and Storage
Store at 4 degree C for 1 month/ -20 degree C for long-term (within shelf life).
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Product Categories/Family for UBA2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
71,224 Da
NCBI Official Full Name
SUMO-activating enzyme subunit 2
NCBI Official Synonym Full Names
ubiquitin-like modifier activating enzyme 2
NCBI Official Symbol
UBA2
NCBI Official Synonym Symbols
ARX; SAE2; HRIHFB2115
NCBI Protein Information
SUMO-activating enzyme subunit 2; SUMO1 activating enzyme subunit 2; SUMO-1 activating enzyme subunit 2; ubiquitin-like 1-activating enzyme E1B; anthracycline-associated resistance ARX; UBA2, ubiquitin-activating enzyme E1 homolog
UniProt Protein Name
SUMO-activating enzyme subunit 2
Protein Family
UniProt Gene Name
UBA2
UniProt Synonym Gene Names
SAE2; UBLE1B
UniProt Entry Name
SAE2_HUMAN
NCBI Description
Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]
Uniprot Description
SAE2: a protein of the ubiquitin-activating E1 family. Acts as a UBL1 E1 ligase. Mediates ATP-dependent activation of UBL1 and formation of a thiolester with a conserved cysteine residue on SAE2.
Protein type: Ligase; EC 6.3.2.19; Ubiquitin conjugating system; EC 6.3.2.-; Motility/polarity/chemotaxis; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 19q12
Cellular Component: nucleoplasm; nucleus; cytosol
Molecular Function: SUMO activating enzyme activity; protein binding; protein heterodimerization activity; metal ion binding; enzyme activator activity; transcription factor binding; ATP binding
Biological Process: positive regulation of catalytic activity; cellular protein metabolic process; protein sumoylation; SMT3-dependent protein catabolic process; post-translational protein modification
Research Articles on UBA2
Similar Products
Product Notes
The Human UBA2 uba2 (Catalog #AAA2709715) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2709715 ELISA Kit recognizes Human UBA2. AAA Biotech's Ubiquitin Like Modifier Activating Enzyme 2 (UBA2) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the UBA2 uba2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ubiquitin Like Modifier Activating Enzyme 2 (UBA2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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