Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ubiquitin-60S ribosomal protein L40 (UBA52) Standard Curve.)
Human Ubiquitin-60S Ribosomal Protein L40 (UBA52) ELISA Kit | UBA52 elisa kit
Human Ubiquitin-60S Ribosomal Protein L40 (UBA52) ELISA Kit
Gene Names
UBA52; UBB; UBC; RPS27A
Reactivity
Human
Synonyms
Ubiquitin-60S Ribosomal Protein L40 (UBA52); Human Ubiquitin-60S Ribosomal Protein L40 (UBA52) ELISA Kit; UBA52; CEP52; HUBCEP52; MGC126879; MGC126881; MGC57125; RPL40; 60S ribosomal protein L40; ribosomal protein L40; ubiquitin and ribosomal protein L40; ubiquitin carboxyl extension protein 52; ubiquitin-52 amino; UBA52 elisa kit
Reactivity
Human
Specificity
Human Ubiquitin-60S ribosomal protein L40 (UBA52) ELISA Kit has high sensitivity and excellent specificity for detection of Human UBA52. No significant cross-reactivity or interference between Human UBA52 and analogues was observed.
Sequence Length
128
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ubiquitin-60S ribosomal protein L40 (UBA52) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ubiquitin-60S ribosomal protein L40 (UBA52) Standard Curve.)
Related Product Information for UBA52 elisa kit
Description: This Human Ubiquitin-60S ribosomal protein L40 (UBA52) ELISA Kit employs a two-site sandwich ELISA to quantitate UBA52 in samples. An antibody specific for UBA52 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UBA52 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBA52 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBA52 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. UBA52 encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome.
Background: Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. UBA52 encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ubiquitin-60S ribosomal protein L40
NCBI Official Synonym Full Names
ubiquitin A-52 residue ribosomal protein fusion product 1
NCBI Official Symbol
UBA52
NCBI Official Synonym Symbols
UBB; UBC; RPS27A
NCBI Protein Information
ubiquitin-60S ribosomal protein L40
UniProt Protein Name
Ubiquitin-60S ribosomal protein L40
UniProt Gene Name
UBA52
UniProt Synonym Gene Names
UBCEP2
UniProt Entry Name
RL40_BOVIN
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Product Notes
The Human UBA52 uba52 (Catalog #AAA9715971) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715971 ELISA Kit recognizes Human UBA52. It is sometimes possible for the material contained within the vial of "Ubiquitin-60S Ribosomal Protein L40 (UBA52), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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