Human Tyrosine-protein kinase Tec ELISA Kit | TEC elisa kit

Human Tyrosine-protein kinase Tec ELISA Kit

Cat. Num. AAA289920

• Gene Names: TEC; PSCTK4
• Reactivity: Human
• Synonyms: Tyrosine-protein kinase Tec; Human Tyrosine-protein kinase Tec ELISA Kit; TEC; PSCTK4; 2.7.10.2; TEC elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human Tyrosine-protein kinase Tec
• Sequence Length: 631

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.156-10 ng/mL
• Sensitivity: < 0.076 ng/mL
• Intra-assay Precision: <=5.8%
• Inter-assay Precision: <=9.3%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TEC elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 130977759
• NCBI GeneID: 7006
• NCBI Accession #: NP_003206.2
• NCBI GenBank Nucleotide #: NM_003215.2
• UniProt Accession #: P42680; Q3MIS5; B7ZKZ6
• Molecular Weight: 73,581 Da
• NCBI Official Full Name: tyrosine-protein kinase Tec
• NCBI Official Synonym Full Names: tec protein tyrosine kinase
• NCBI Official Symbol: TEC
• NCBI Official Synonym Symbols: PSCTK4
• NCBI Protein Information: tyrosine-protein kinase Tec
• UniProt Protein Name: Tyrosine-protein kinase Tec
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: TEC
• UniProt Synonym Gene Names: PSCTK4
• UniProt Entry Name: TEC_HUMAN

NCBI Description

The protein encoded by this gene belongs to the Tec family of non-receptor protein-tyrosine kinases containing a pleckstrin homology domain. Tec family kinases are involved in the intracellular signaling mechanisms of cytokine receptors, lymphocyte surface antigens, heterotrimeric G-protein coupled receptors, and integrin molecules. They are also key players in the regulation of the immune functions. Tec kinase is an integral component of T cell signaling and has a distinct role in T cell activation. This gene may be associated with myelodysplastic syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

TEC: a non-receptor protein-tyrosine kinases of the Tec family. Containing a pleckstrin homology domain. Involved in the intracellular signaling mechanisms of cytokine receptors, lymphocyte surface antigens, heterotrimeric G-protein coupled receptors, and integrin molecules. May be an important signal transducer for cell division and/or for differentiation in the liver system.

Protein type: EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; TK group; Tec family

Chromosomal Location of Human Ortholog: 4p12

Cellular Component: cytoskeleton; cytosol; extrinsic to internal side of plasma membrane; intercellular junction

Molecular Function: ATP binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; phospholipid binding; protein binding; receptor binding

Biological Process: adaptive immune response; B cell receptor signaling pathway; cell differentiation; innate immune response; integrin-mediated signaling pathway; peptidyl-tyrosine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; protein amino acid phosphorylation; regulation of cell proliferation; tissue regeneration; transmembrane receptor protein tyrosine kinase signaling pathway