Human Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Epsilon (YWHAe) ELISA Kit | YWHAe elisa kit

Human Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Epsilon (YWHAe) ELISA Kit

Cat. Num. AAA2122210

• Gene Names: YWHAE; MDS; HEL2; MDCR; KCIP-1; 14-3-3E
• Reactivity: Human
• Synonyms: Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Epsilon (YWHAe); Human Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Epsilon (YWHAe) ELISA Kit; MDCR; MDS; KCIP-1; 14-3-3E; 14-3-3 protein epsilon; YWHAe elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Epsilon (YWHAe).; No significant cross-reactivity or interference between Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Epsilon (YWHAe) and analogues was observed.
• Sequence Length: 255

• Assay Type: Double-antibody Sandwich
• Sample: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Dectection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.054ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Epsilon (YWHAe) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Epsilon (YWHAe) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for YWHAe elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Epsilon (YWHAe). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Epsilon (YWHAe). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Epsilon (YWHAe), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Epsilon (YWHAe) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for YWHAe elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 5803225
• NCBI GeneID: 7531
• NCBI Accession #: NP_006752.1
• NCBI GenBank Nucleotide #: NP_006752.1
• UniProt Accession #: P62258
• NCBI Official Full Name: 14-3-3 protein epsilon
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon
• NCBI Official Symbol: YWHAE
• NCBI Official Synonym Symbols: MDS; HEL2; MDCR; KCIP-1; 14-3-3E
• NCBI Protein Information: 14-3-3 protein epsilon
• UniProt Protein Name: 14-3-3 protein epsilon
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAE
• UniProt Synonym Gene Names: 14-3-3E
• UniProt Entry Name: 1433E_HUMAN

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

14-3-3 epsilon: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: kinesin complex; cytoplasmic vesicle membrane; focal adhesion; mitochondrion; membrane; axon; melanosome; cytosol

Molecular Function: protein domain specific binding; protein binding; enzyme binding; potassium channel regulator activity; protein heterodimerization activity; histone deacetylase binding; phosphoprotein binding; phosphoserine binding

Biological Process: substantia nigra development; nerve growth factor receptor signaling pathway; viral reproduction; apoptosis; organelle organization and biogenesis; hippocampus development; neuron migration; mitotic cell cycle; cerebral cortex development; regulation of caspase activity; G2/M transition of mitotic cell cycle; regulation of the rate of heart contraction by hormone; protein targeting

Disease: Miller-dieker Lissencephaly Syndrome