Human Twisted Gastrulation Protein Homolog 1 (TWSG1) ELISA Kit | TWSG1 elisa kit

Human Twisted Gastrulation Protein Homolog 1 (TWSG1) ELISA Kit

Cat. Num. AAA451568

• Gene Names: TWSG1; TSG
• Reactivity: Human
• Synonyms: Twisted Gastrulation Protein Homolog 1 (TWSG1); Human Twisted Gastrulation Protein Homolog 1 (TWSG1) ELISA Kit; B-CK; CKBB; Creatine kinase B chain; Creatine kinase B-type; TWSG1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TWSG1. No significant cross-reactivity or interference between TWSG1 and analogues was observed.
• Sequence Length: 223

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.112ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TWSG1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TWSG1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TWSG1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TWSG1 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TWSG1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TWSG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TWSG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TWSG1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 10190664
• NCBI GeneID: 57045
• NCBI Accession #: NP_065699.1
• NCBI GenBank Nucleotide #: NM_020648.5
• UniProt Accession #: Q9GZX9; Q8NBI7; Q96K46; B2RE08; D3DUH9
• Molecular Weight: 8,284 Da
• NCBI Official Full Name: twisted gastrulation protein homolog 1
• NCBI Official Synonym Full Names: twisted gastrulation BMP signaling modulator 1
• NCBI Official Symbol: TWSG1
• NCBI Official Synonym Symbols: TSG
• NCBI Protein Information: twisted gastrulation protein homolog 1
• UniProt Protein Name: Twisted gastrulation protein homolog 1
• Protein Family: Twisted gastrulation protein
• UniProt Gene Name: TWSG1
• UniProt Synonym Gene Names: TSG

Uniprot Description

May be involved in dorsoventral axis formation. Seems to antagonize BMP signaling by forming ternary complexes with CHRD and BMPs, thereby preventing BMPs from binding to their receptors. In addition to the anti-BMP function, also has pro-BMP activity, partly mediated by cleavage and degradation of CHRD, which releases BMPs from ternary complexes. May be an important modulator of BMP-regulated cartilage development and chondrocyte differentiation. May play a role in thymocyte development ().