Human Twinfilin 1 (TWF1) ELISA Kit | TWF1 elisa kit

Human Twinfilin 1 (TWF1) ELISA Kit

Cat. Num. AAA453319

• Gene Names: TWF1; A6; PTK9
• Reactivity: Human
• Synonyms: Twinfilin 1 (TWF1); Human Twinfilin 1 (TWF1) ELISA Kit; A6; PTK9; Actin-Binding Protein; Homolog 1; Protein Tyrosine Kinase 9; TWF1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TWF1. No significant cross-reactivity or interference between TWF1 and analogues was observed.
• Sequence Length: 357

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.054ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TWF1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TWF1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TWF1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TWF1 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TWF1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TWF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TWF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TWF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 334848198
• NCBI GeneID: 5756
• NCBI Accession #: NP_001229326.1
• NCBI GenBank Nucleotide #: NM_001242397.1
• UniProt Accession #: Q12792; Q59G07; Q5U0B1; Q6FHJ1; Q6FHL6; Q6NUK9; Q86XL6; Q8TCD3; A8K5A8; B3KXS6; B4DLX9
• Molecular Weight: 28,823 Da
• NCBI Official Full Name: twinfilin-1 isoform 1
• NCBI Official Synonym Full Names: twinfilin actin binding protein 1
• NCBI Official Symbol: TWF1
• NCBI Official Synonym Symbols: A6; PTK9
• NCBI Protein Information: twinfilin-1
• UniProt Protein Name: Twinfilin-1
• Protein Family: Twinfilin
• UniProt Gene Name: TWF1
• UniProt Synonym Gene Names: PTK9

NCBI Description

This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1. [provided by RefSeq, Jul 2008]

Uniprot Description

Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles ().