Typical Testing Data/Standard Curve (for reference only)
Human Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4) ELISA Kit | TNFRSF4 elisa kit
Human Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4)(Sandwich ELISA) ELISA Kit
Reactivity
Human
Applications
ELISA
Synonyms
Tumor Necrosis Factor Receptor Superfamily; Member 4 (TNFRSF4); Human Tumor Necrosis Factor Receptor Superfamily; Member 4 (TNFRSF4)(Sandwich ELISA) ELISA Kit; ELISA Kit for Tumor Necrosis Factor Receptor Superfamily; TNFRSF4/Tumor Necrosis Factor Receptor Superfamily; Member 4; CD134; TNFRSF4; ACT35; OX40; TXGP1L; TAX transcriptionally-activated glycoprotein 1 receptor; TNFRSF4 elisa kit
Reactivity
Human
Specificity
Reactive with Human Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4)
This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4). No significant cross-reactivity or interference between Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4) and analogues was observed.
This assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4). No significant cross-reactivity or interference between Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4) and analogues was observed.
Applicable Applications for TNFRSF4 elisa kit
ELISA (EIA)
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Assay Type
Sandwich ELISA
Sample Types
tissue homogenates, cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.061ng/mL
Detection Method
Double-antibody Sandwich
Assay Length
3 hours
Assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100.
Intra-Assay: CV<10%.
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100.
Intra-Assay: CV<10%.
Inter-Assay: CV<12%
Test Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor Binding Protein 6 (IGFBP6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor Binding Protein 6 (IGFBP6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor Binding Protein 6 (IGFBP6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Insulin Like Growth Factor Binding Protein 6 (IGFBP6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Procedure Summary
1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 50ul Stop Solution. Read at 450nm immediately.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
8. Add 50ul Stop Solution. Read at 450nm immediately.
Preparation and Storage
Store at 4 degree C for 1 month; -20 degree C for long-term (within shelf life)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Product Categories/Family for TNFRSF4 elisa kit
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Product Notes
The Human TNFRSF4 (Catalog #AAA2125246) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2125246 ELISA Kit recognizes Human TNFRSF4. AAA Biotech's Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the TNFRSF4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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