Human Trefoil Factor 3 (TFF3) ELISA Kit | TFF3 elisa kit

Human Trefoil Factor 3 (TFF3) High Sensitive ELISA Kit

Cat. Num. AAA2708066

• Gene Names: TFF3; ITF; P1B; TFI
• Reactivity: Human
• Applications: ELISA
• Synonyms: Trefoil Factor 3 (TFF3); Human Trefoil Factor 3 (TFF3) High Sensitive ELISA Kit; Trefoil Factor 3; HITF; ITF3; TFI; HP1.B; Intestinal; TFF3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Trefoil Factor 3 (TFF3).; No significant cross-reactivity or interference between High Sensitive Trefoil Factor 3 (TFF3) and analogues was observed.
• Sequence Length: 94

• Applicable Applications for TFF3 elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 31.2-2,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 14.3pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Trefoil Factor 3 (TFF3) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Trefoil Factor 3 (TFF3) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Trefoil Factor 3 (TFF3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Trefoil Factor 3 (TFF3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Trefoil Factor 3 (TFF3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Trefoil Factor 3 (TFF3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 281485608
• NCBI GeneID: 7033
• NCBI Accession #: NP_003217.3
• NCBI GenBank Nucleotide #: NP_003217.3
• UniProt Accession #: Q07654
• NCBI Official Full Name: trefoil factor 3
• NCBI Official Synonym Full Names: trefoil factor 3
• NCBI Official Symbol: TFF3
• NCBI Official Synonym Symbols: ITF; P1B; TFI
• NCBI Protein Information: trefoil factor 3
• UniProt Protein Name: Trefoil factor 3
• Protein Family: Trefoil factor
• UniProt Gene Name: TFF3
• UniProt Synonym Gene Names: ITF; TFI; hITF; hP1.B
• UniProt Entry Name: TFF3_HUMAN

NCBI Description

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]

Uniprot Description

TFF3: Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular region; secretory granule

Biological Process: response to peptide hormone stimulus; digestion; defense response