Human Transmembrane Protease, Serine 2 (TMPRSS2) ELISA Kit | TMPRSS2 elisa kit

Human Transmembrane Protease, Serine 2 (TMPRSS2) ELISA Kit

Cat. Num. AAA2019750

• Gene Names: TMPRSS2; PP9284; PRSS10
• Reactivity: Human
• Synonyms: Transmembrane Protease; Serine 2 (TMPRSS2); Human Transmembrane Protease; Serine 2 (TMPRSS2) ELISA Kit; PRSS10; Serine protease 10; TMPRSS2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TMPRSS2. No significant cross-reactivity or interference between TMPRSS2 and analogues was observed.
• Sequence Length: 492

• Samples: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TMPRSS2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TMPRSS2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TMPRSS2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TMPRSS2 in human serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TMPRSS2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TMPRSS2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TMPRSS2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TMPRSS2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TMPRSS2 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 30353995
• NCBI GeneID: 7113
• UniProt Accession #: O15393; Q6GTK7; Q9BXX1; A8K6Z8; B2R8E5; B7Z459; D3DSJ2; F8WES1
• Molecular Weight: 57,690 Da
• NCBI Official Full Name: Transmembrane protease, serine 2
• NCBI Official Synonym Full Names: transmembrane protease, serine 2
• NCBI Official Symbol: TMPRSS2
• NCBI Official Synonym Symbols: PP9284; PRSS10
• NCBI Protein Information: transmembrane protease serine 2
• UniProt Protein Name: Transmembrane protease serine 2
• Protein Family: Transmembrane protease serine
• UniProt Gene Name: TMPRSS2
• UniProt Synonym Gene Names: PRSS10
• UniProt Entry Name: TMPS2_HUMAN

NCBI Description

This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

TMPRSS2: a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Protein type: EC 3.4.21.-; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity; scavenger receptor activity

Biological Process: protein autoprocessing; receptor-mediated endocytosis; positive regulation of virion penetration into host cell; proteolysis