Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Translocating chain-associated membrane protein 1 (TRAM1) Standard Curve.)
Human Translocating Chain-Associated Membrane Protein 1 (TRAM1) ELISA Kit | TRAM1 elisa kit
Human Translocating Chain-Associated Membrane Protein 1 (TRAM1) ELISA Kit
Gene Names
TRAM1; TRAM; PNAS8; TRAMP
Reactivity
Human
Synonyms
Translocating Chain-Associated Membrane Protein 1 (TRAM1); Human Translocating Chain-Associated Membrane Protein 1 (TRAM1) ELISA Kit; TRAM1; PNAS8; PRO1292; TRAM; TRAMP; translocating chain-associating membrane protein; translocation-associating membrane protein 1; TRAM1 elisa kit
Reactivity
Human
Specificity
Human Translocating chain-associated membrane protein 1 (TRAM1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TRAM1. No significant cross-reactivity or interference between Human TRAM1 and analogues was observed.
Sequence Length
374
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Translocating chain-associated membrane protein 1 (TRAM1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Translocating chain-associated membrane protein 1 (TRAM1) Standard Curve.)
Related Product Information for TRAM1 elisa kit
Description: This Human Translocating chain-associated membrane protein 1 (TRAM1) ELISA Kit employs a two-site sandwich ELISA to quantitate TRAM1 in samples. An antibody specific for TRAM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRAM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRAM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRAM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TRAM1 is a multi-pass membrane protein that is part of the mammalian endoplasmic reticulum. The protein influences glycosylation and facilitates the translocation of secretory proteins across the endoplasmic reticulum membrane by regulating which domains of the nascent polypeptide chain are visible to the cytosol during a translocational pause. TRAM is a 374-amino acid, 8-pass transmembrane protein that shares 95% amino acid identity with the canine protein. TRAM contains a potential N-terminal N-linked glycosylation site and a C-terminal 60-residue cytosolic portion. Immunoblot analysis showed that TRAM is an abundantly expressed, approximately 36-kD microsomal glycoprotein. Functional analysis indicated that TRAM influences glycosylation and is stimulatory or required for the translocation of secretory proteins.
Background: TRAM1 is a multi-pass membrane protein that is part of the mammalian endoplasmic reticulum. The protein influences glycosylation and facilitates the translocation of secretory proteins across the endoplasmic reticulum membrane by regulating which domains of the nascent polypeptide chain are visible to the cytosol during a translocational pause. TRAM is a 374-amino acid, 8-pass transmembrane protein that shares 95% amino acid identity with the canine protein. TRAM contains a potential N-terminal N-linked glycosylation site and a C-terminal 60-residue cytosolic portion. Immunoblot analysis showed that TRAM is an abundantly expressed, approximately 36-kD microsomal glycoprotein. Functional analysis indicated that TRAM influences glycosylation and is stimulatory or required for the translocation of secretory proteins.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Translocating chain-associated membrane protein 1
NCBI Official Synonym Full Names
translocation associated membrane protein 1
NCBI Official Symbol
TRAM1
NCBI Official Synonym Symbols
TRAM; PNAS8; TRAMP
NCBI Protein Information
translocating chain-associated membrane protein 1
UniProt Protein Name
Translocating chain-associated membrane protein 1
Protein Family
UniProt Gene Name
TRAM1
UniProt Synonym Gene Names
TRAM
UniProt Entry Name
TRAM1_HUMAN
NCBI Description
This gene encodes a multi-pass membrane protein that is part of the mammalian endoplasmic reticulum. The encoded protein influences glycosylation and facilitates the translocation of secretory proteins across the endoplasmic reticulum membrane by regulating which domains of the nascent polypeptide chain are visible to the cytosol during a translocational pause. [provided by RefSeq, Oct 2009]
Uniprot Description
TRAM1: Stimulatory or required for the translocation of secretory proteins across the ER membrane. Belongs to the TRAM family.
Protein type: Membrane protein, multi-pass; Receptor, misc.; Transporter; Membrane protein, integral
Chromosomal Location of Human Ortholog: 8q13.3
Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane
Molecular Function: protein binding; receptor activity
Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translation; viral reproduction; gene expression; cotranslational protein targeting to membrane
Research Articles on TRAM1
Similar Products
Product Notes
The Human TRAM1 tram1 (Catalog #AAA9713805) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713805 ELISA Kit recognizes Human TRAM1. It is sometimes possible for the material contained within the vial of "Translocating Chain-Associated Membrane Protein 1 (TRAM1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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