Human Transglutaminase 2, Tissue (TGM2) ELISA Kit | TGM2 elisa kit

Human Transglutaminase 2, Tissue (TGM2) ELISA Kit

Cat. Num. AAA2020700

• Gene Names: TGM2; TG2; TGC; GNAH; HEL-S-45; G-ALPHA-h
• Reactivity: Human
• Synonyms: Transglutaminase 2; Tissue (TGM2); Human Transglutaminase 2; Tissue (TGM2) ELISA Kit; tTG; TG2; C Polypeptide; Protein-Glutamine-Gamma-Glutamyltransferase; Transglutaminase C; Transglutaminase H; TGM2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Transglutaminase 2, Tissue (TGM2).; No significant cross-reactivity or interference between Transglutaminase 2, Tissue (TGM2) and analogues was observed.
• Sequence Length: 687

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.625-40ng/mL
• Sensitivity: < 0.249ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transglutaminase 2, Tissue (TGM2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transglutaminase 2, Tissue (TGM2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TGM2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transglutaminase 2, Tissue (TGM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transglutaminase 2, Tissue (TGM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transglutaminase 2, Tissue (TGM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transglutaminase 2, Tissue (TGM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TGM2 elisa kit

Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 50593093
• NCBI GeneID: 7052
• UniProt Accession #: P21980; Q16436; Q6B838; Q9BTN7; Q9UH35; E1P5V9
• Molecular Weight: 38,671 Da
• NCBI Official Full Name: transglutaminase 2
• NCBI Official Synonym Full Names: transglutaminase 2
• NCBI Official Symbol: TGM2
• NCBI Official Synonym Symbols: TG2; TGC; GNAH; HEL-S-45; G-ALPHA-h
• NCBI Protein Information: protein-glutamine gamma-glutamyltransferase 2
• UniProt Protein Name: Protein-glutamine gamma-glutamyltransferase 2
• Protein Family: Protein-glutamine gamma-glutamyltransferase
• UniProt Gene Name: TGM2
• UniProt Synonym Gene Names: TG(C); TGC; TGase C; TGase H; TGase-2
• UniProt Entry Name: TGM2_HUMAN

NCBI Description

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TGM2: an enzyme of the transglutaminase family that catalyzes the crosslinking of proteins and the conjugation of polyamines to proteins. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. . Is the autoantigen in coeliac disease and plays a role in apoptosis, cellular differentiation and matrix stabilisation. Three alternatively spliced isoforms have been described.

Protein type: EC 2.3.2.13; Transferase

Chromosomal Location of Human Ortholog: 20q12

Cellular Component: focal adhesion; mitochondrion; plasma membrane; cytosol

Molecular Function: protein domain specific binding; protein binding; GTP binding; protein-glutamine gamma-glutamyltransferase activity; metal ion binding

Biological Process: elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of cell adhesion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of apoptosis; isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine; positive regulation of smooth muscle cell proliferation; reduction of endoplasmic reticulum calcium ion concentration; blood vessel remodeling; apoptotic cell clearance; protein homooligomerization; positive regulation of inflammatory response; elevation of mitochondrial calcium ion concentration