Human TPO ELISA Kit | TPO elisa kit

Anti-TPO ELISA

Cat. Num. AAA494668

• Gene Names: TPO; MSA; TPX; TDH2A
• Reactivity: Human
• Synonyms: TPO; Anti-TPO ELISA; TPO elisa kit

• Reactivity: Human
• Specificity: The microplate is coated with antigen, isolated from human thyroid tissue and highly purified by affinity chromatography. The Anti-TPO is a specific test kit for the quantitation of IgG-class autoantibodies directed against human thyroid peroxidase (TPO). The antigen used in the test kit is purified to exhibit high activity of thyroid peroxidase.
• Sequence Length: 876

• Samples: Human serum or plasma
• Sensitivity: The lower detection limit for Anti-TPO ELISA was determined at 5 IU/ml.
• Intended Uses: Anti-TPO is an indirect solid phase enzyme immunoassay (ELISA) for the quantitative measurement of IgG class autoantibodies against thyroid peroxidase (TPO) in human serum or plasma.
• Preparation and Storage: 2 - 8 degree C

Related Product Information for TPO elisa kit

Principle of the assay: Highly purified human thyroid peroxidase (TPO) is bound to microwells. Antibodies to this antigen, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated anti-human IgG immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow end-product. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.

Product Categories/Family for TPO elisa kit

Autoimmune; Immunology; Assay Categories

NCBI and Uniprot Product Information

• NCBI GI #: 332078492
• NCBI GeneID: 7173
• NCBI Accession #: NP_001193674.1
• NCBI GenBank Nucleotide #: NM_001206745.1
• Molecular Weight: 92,063 Da
• NCBI Official Full Name: thyroid peroxidase isoform b
• NCBI Official Synonym Full Names: thyroid peroxidase
• NCBI Official Symbol: TPO
• NCBI Official Synonym Symbols: MSA; TPX; TDH2A
• NCBI Protein Information: thyroid peroxidase; thyroid microsomal antigen; thyroperoxidase
• UniProt Protein Name: Thyroid peroxidase
• Protein Family: Thyroid peroxidase
• UniProt Gene Name: TPO
• UniProt Synonym Gene Names: TPO
• UniProt Entry Name: PERT_HUMAN

NCBI Description

This gene encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2011]

Uniprot Description

TPO: Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4). An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease. Defects in TPO are the cause of thyroid dyshormonogenesis 2A (TDH2A). A disorder due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete. Belongs to the peroxidase family. XPO subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - tyrosine; Mitochondrial; Membrane protein, integral; Oxidoreductase; EC 1.11.1.8

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: extracellular space; cell surface; mitochondrion; integral to plasma membrane; plasma membrane

Molecular Function: peroxidase activity; heme binding; iodide peroxidase activity; calcium ion binding

Biological Process: hydrogen peroxide catabolic process; embryonic hemopoiesis; thyroid hormone generation; response to oxidative stress; hormone biosynthetic process

Disease: Thyroid Dyshormonogenesis 2a